The amphoterin-induced gene and ORF (AMIGO) family of proteins consists of AMIGO-1, AMIGO-2 and AMIGO-3. All three members are single pass type I membrane proteins that contain several leucine-rich repeats, one IgG domain, and a transmembrane domain. The AMIGO proteins are specifically expressed on fiber tracts of neuronal tissues and participate in their formation. The AMIGO proteins can form complexes with each other, but can also bind itself. AMIGO-1, also designated Alivin-2, promotes growth and fasciculation of neurites and plays a role in myelination and fasciculation of developing neural axons. In cerebellar neurons, AMIGO-2 (Alivin-1) is crucial for depolarization-dependent survival. Similar to AMIGO-1 and AMIGO-2, AMIGO-3 (Alivin-3) plays a role in homophilic and/or heterophilic cell-cell interaction and signal transduction.
Function:
AMIGO1 promotes growth and fasciculation of neurites from cultured hippocampal neurons. It may be involved in fasciculation as well as myelination of developing neural axons and have a role in regeneration as well as neural plasticity in the adult nervous system. AMIGO1 may also mediate homophilic as well as heterophilic cell-cell interaction and contribute to signal transduction through its intracellular domain.
Subunit:
Homodimer, and heterodimer with AMIGO2 and AMIGO3.
Subcellular Location:
Membrane; Single-pass type I membrane protein. Cell projection; axon. Note: Associated with axons of neuronal cells.
Similarity:
Belongs to the immunoglobulin superfamily. AMIGO family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 6 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain.
SWISS:
Q86WK6
Gene ID:
57463
Database links:
Entrez Gene: 57463 Human
Entrez Gene: 229715 Mouse
Entrez Gene: 295365 Rat
SwissProt: Q86WK6 Human
SwissProt: Q80ZD8 Mouse
SwissProt: Q80ZD7 Rat
Unigene: 726479 Human
Unigene: 275752 Mouse
Unigene: 116338 Rat
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Sample:
Hippocampus (Mouse) Lysate at 40 ug
Cerebral cortex (Mouse) Lysate at 40 ug
Cerebellum (Mouse) Lysate at 40 ug
Primary: Anti-AMIGO1 (SL11070R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD
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