Rabbit Anti-Progestin Receptor Beta antibody

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MPRB; C6orf33; LMPB1; Lysosomal membrane protein in brain 1; Membrane progestin receptor beta; mPR beta; MPRB; PAQR8; Progestin and adipoQ receptor family member 8; Progestin and adipoQ receptor family member VIII; MPRB_HUMAN.

Cat:

SL11410R

Species Reactivity：

Human,Mouse,Rat,Dog,(predicted: Chicken,Pig,Cow,Horse,Rabbit,Sheep,.)

Immunogen：

KLH conjugated synthetic peptide derived from human Progestin Receptor Beta:251-300/354<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Hippocampus (Mouse) Lysate at 40 ugLane 2: Medulld (Mouse) Lysate at 40 ugLane 3: Hippocampus (Rat) Lysate at 40 ugLane 4: Medulld (Rat) Lysate at 40 ugPrimary: Anti-Progestin Receptor Beta (SL11410R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 42 kDObserved band size: 46 kD Paraformaldehyde-fixed, paraffin embedded (Dog skin tumor); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Progestin Receptor Beta) Polyclonal Antibody, Unconjugated (SL11410R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The steroid progesterone induces the resumption of maturation in oocytes via a nongenomic pathway through binding to a novel membrane progestin receptor (mPR). This pathway inhibits adenylyl cyclase and reduces intracellular cAMP, and also activates mitogen-activated protein kinase to effect signal transduction pathways. Five distinct groups, designated Alpha, Beta, Gamma, Delta, comprise the mPR gene family. mPR Alpha, also designated progestin and adipo Theta receptor family member VII (PAQR7), consists of an extracellular N-terminus, an intracellular SLCterminus and seven transmembrane domains. mPR Alpha is expressed in ovary, testis, placenta, uterus and bladder. mPR Beta, or progestin and adipo Theta receptor family member VIII (PAQR8), consists of eight putative transmembrane regions and an intracellular N-terminus that contains a leucine-rich motif. mPR Beta is a 354 amino acid protein expressed in brain and spinal cord. Both mPR Alpha and mPR Beta may be G protein-coupled receptors and may be involved in oocyte maturation.

Function:
MPRb (Membrane progestin receptor beta) is a steroid membrane receptor and binds progesterone. MPRb may be involved in oocyte maturation. MPRb is an integral membrane protein and is expressed in brain and testis. It belongs to the ADIPOR family.

Subcellular Location:
Cell Membrane. Multi-pass membrane protein. Note: Colocalizes with a lysosomal protein cathepsin D.

Similarity:
Belongs to the ADIPOR family.

SWISS:
Q8TEZ7

Gene ID:
85315

Database links:

Entrez Gene: 85315 Human

Entrez Gene: 74229 Mouse

Entrez Gene: 316275 Rat

Omim: 607780 Human

SwissProt: Q8TEZ7 Human

SwissProt: Q80ZE5 Mouse

SwissProt: Q6AY03 Pig

Unigene: 239388 Human

Picture

Paraformaldehyde-fixed, paraffin embedded (Dog skin tumor); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Progestin Receptor Beta) Polyclonal Antibody, Unconjugated (SL11410R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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