ADP-ribosylation factor (ARF) family of small GTP-binding proteins are involved in vesicular transport regulation and in controlling cytoskeletal organization and cell adhesion. These proteins are best characterized as regulators of membrane traffic. The Centaurin GTPase-activating protein family comprise a subset of ARF regulatory molecules that transduce PI 3-kinase activation into coordinated control of Arf-dependent pathways. This family includes ASAP1, ACAP1, ACAP2, AGAP1, ARAP1, ARAP2, Centaurin a1 and Centaurin g3. The Centaurin a1 protein is a high affinity PtdIns(3,4,5)P3 binding protein enriched in brain. By acting as a GTPase activating protein for ADP ribosylation factor 6 (ARF6), Centaurin a1 is able to switch off ARF6 and inhibit its ability to mediate b2-adrenoceptor internalization and negatively regulate ARF6 activity by functioning as an in vivo PIP3 dependent ARF6 GAP.
Function:
The centaurin alpha 1 protein is a high affinity PtdIns(3,4,5)P3 binding protein enriched in brain. By acting as a GTPase activating protein for ADP ribosylation factor 6 (ARF6), centaurin alpha 1 is able to switch off ARF6 and inhibit its ability to mediate beta 2 adrenoceptor internalization and negatively regulates ARF6 activity by functioning as an in vivo PIP3 dependent ARF6 GAP.
Subunit:
Interacts with PRKCA, PRKCI and PRKCZ. Interacts with the N-terminal region of PRKD1.
Subcellular Location:
Cellular localizationCytoplasmic, Nuclear and Plasma membrane
Tissue Specificity:
Expressed at highest levels in brain and at lower levels in peripheral blood leukocytes.
Post-translational modifications:
Phosphorylated by PRKCA, PRKCI, PRKCZ and PRKD1 in vitro.
Similarity:
Contains 1 Arf-GAP domain.
Contains 2 PH domains.
SWISS:
O75689
Gene ID:
11033
Database links:
Entrez Gene: 11033 Human
Entrez Gene: 231821 Mouse
Entrez Gene: 397453 Pig
Entrez Gene: 171097 Rat
Omim: 608114 Human
SwissProt: O75689 Human
Unigene: 297819 Mouse
Unigene: 10539 Rat
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Tissue/cell: Rat brain ; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Centaurin alpha 1 Polyclonal Antibody, Unconjugated(SL11543R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
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