[email protected]
Home > Product > Antibody > Rabbit Anti-NCS1 antibody
9430075O15Rik; A730032G13Rik; AI836659; DKFZp761L1223; FLUP; Frequenin; Frequenin homolog (Drosophila); Frequenin homolog; Frequenin like protein; Frequenin, Drosophila, homolog of; Frequenin-like protein; Frequenin-like ubiquitous protein; Mfreq; NCS 1;
Cat:
SL11977R
Species Reactivity：
Rat,(predicted: Human,Mouse,Cow,)
Immunogen：
KLH conjugated synthetic peptide derived from human NCS1:1-50/190
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NCS1) Polyclonal Antibody, Unconjugated (SL11977R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
More
prev next
Unit:
Price: $
Product PDFs
Datasheet:


This gene is a member of the neuronal calcium sensor gene family, which encode calcium-binding proteins expressed predominantly in neurons. The protein encoded by this gene regulates G protein-coupled receptor phosphorylation in a calcium-dependent manner and can substitute for calmodulin. The protein is associated with secretory granules and modulates synaptic transmission and synaptic plasticity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Function:
Neuronal calcium sensor, regulator of G protein-coupled receptor phosphorylation in a calcium dependent manner. Directly regulates GRK1 (RHOK), but not GRK2 to GRK5. Can substitute for calmodulin (By similarity). Stimulates PI4KB kinase activity (By similarity). Involved in long-term synaptic plasticity through its interaction with PICK1 (By similarity). May also play a role in neuron differentiation through inhibition of the activity of N-type voltage-gated calcium channel.

Subunit:
Interacts with KCND2. Interacts in a calcium-independent manner with PI4KB. This binding competes with CALN2/CABP7 binding to PI4KB (By similarity). Interacts with ARF1, ARF3, ARF5 and ARF6. Interacts in a calcium-dependent manner with PICK1 (via AH domain) (By similarity). Interacts with IL1RAPL1.

Subcellular Location:
Golgi apparatus, Golgi stack membrane. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cytoplasm, perinuclear region. Cell membrane. Associated with Golgi stacks. Post-synaptic densities of dendrites, and in the pre-synaptic nerve terminal at neuromuscular junctions.

Similarity:
Belongs to the recoverin family.
Contains 4 EF-hand domains.

SWISS:
P62166

Gene ID:
23413

Database links:

Entrez Gene: 23413 Human

Entrez Gene: 14299 Mouse

Entrez Gene: 65153 Rat

Omim: 603315 Human

SwissProt: P62166 Human

SwissProt: Q8BNY6 Mouse

SwissProt: P62168 Rat

Unigene: 642946 Human

Unigene: 249318 Mouse

Unigene: 62653 Rat



Picture

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NCS1) Polyclonal Antibody, Unconjugated (SL11977R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Product Feedback Wall
Message :
Your Email :
Copyright © 2007-2018 Sunlong Medical All Rights Reserved.