Rabbit Anti-FLRT3 antibody | Sunlong Medical

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FLRT3_HUMAN; Leucine-rich repeat transmembrane protein FLRT3; KIAA1469; Fibronectin-like domain-containing leucine-rich transmembrane protein 3; UNQ856/PRO1865;

Cat:

SL12363R

Species Reactivity：

Rat,(predicted: Human,Mouse,Chicken,Dog,Pig,Cow,Horse,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human FLRT3:415-490/649<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Rat Cerebrum tissue lysates Lane 2: Rat Lung tissue lysates Primary: Anti-FLRT3 (SL12363R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 70 kDaObserved band size: 73 kDaTissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-FLRT3 Polyclonal Antibody, Unconjugated(SL12363R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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FLRT3 is a 649 amino acid single-pass type I membrane protein that contains one fibronectin type-III domain and ten leucine-rich repeats and belongs to the fibronectin leucine rich transmembrane protein (FLRT) family. Expressed in heart, liver, lung, kidney, pancreas, brain, placenta and skeletal muscle, FLRT3 is thought to be involved in receptor signaling events and may play a role in both cell adhesion neurite outgrowth. Defects in the gene encoding mouse FLRT3 may lead to ventral closure, headfold fusion and endoderm migration defects, suggesting that FLRT3 is important for proper cell differentiation and development. FLRT3 exists as multiple alternatively spliced isoforms that are encoded by a gene which maps to human chromosome 20.

Function:
May have a function in cell adhesion and/or receptor signaling.

Subcellular Location:
Membrane; Single-pass type I membrane protein (Probable).

Tissue Specificity:
Expressed in kidney, brain, pancreas, skeletal muscle, lung, liver, placenta, and heart.

Similarity:
Contains 1 fibronectin type-III domain.
Contains 10 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain.

SWISS:
Q9NZU0

Gene ID:
23767

Database links:

Entrez Gene: 428552 Chicken

Entrez Gene: 23767 Human

Entrez Gene: 71436 Mouse

Entrez Gene: 366205 Rat

SwissProt: F1NUK7 Chicken

SwissProt: Q9NZU0 Human

SwissProt: B1H234 Rat

Picture

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-FLRT3 Polyclonal Antibody, Unconjugated(SL12363R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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