Rabbit Anti-phospho-AQP2 (Ser256) antibody

Home > Product > Antibody > Rabbit Anti-phospho-AQP2 (Ser256) antibody

Aquaporin 2 (phospho S256); Aquaporin 2 (phospho Ser256); p-Aquaporin 2 (phospho S256); p-Aquaporin 2 (S256); ADH water channel; AQP 2; AQP CD; AQP2; AQPCD; Aquaporin 2 collecting duct; Aquaporin CD; Aquaporin2; Aquaporine 2; Collecting duct water channel

Cat:

SL12507R

Species Reactivity：

Human,Dog,(predicted: Mouse,Rat,Chicken,Pig,Cow,Horse,Rabbit,Monkey,)

Immunogen：

KLH conjugated synthesised phosphopeptide derived from human AQP2 around the phosphorylation site of Ser256:RQ(p-S)VE

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: MDCK(Dog) Cell Lysate at 30 ugPrimary: Anti-phospho-AQP2 (Ser256) (SL12507R) at 1/500 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 29 kDObserved band size: 29 kDParaformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-AQP2 (Ser256)) Polyclonal Antibody, Unconjugated (SL12507R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

prev next

Unit:

Price: $256.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

Aquaporins (AQPs) are a large family of integral membrane water transport channel proteins that facilitate the transport of water through the cell membrane. This function is conserved in animals, plants and bacteria. Many isoforms of aquaporin have been identified in mammals, designated AQP0 through AQP10. Aquaporins are widely distributed and it is not uncommon for more than one type of AQP to be present in the same cell. Although most aquaporins are only permeable to water, AQP3, AQP7, AQP9 and one of the two AQP10 transcripts are also permeable to urea and glycerol. AQP2 is the only water channel that is activated by vasopressin to enhance water reabsorption in the kidney collecting duct. Aquaporins are involved in renal water absorption, generation of pulmonary secretions, lacrimation, and the secretion and reabsorption of cerebrospinal fluid and aqueous humor.

Function:
Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.

Subcellular Location:
Apical cell membrane. Cytoplasmic vesicle membrane. Shuttles from vesicles to the apical membrane.

Tissue Specificity:
Expressed in renal collecting tubules.

Post-translational modifications:
Ser-256 phosphorylation is necessary and sufficient for expression at the apical membrane. Endocytosis is not phosphorylation-dependent.

DISEASE:
Defects in AQP2 are the cause of diabetes insipidus nephrogenic autosomal (ANDI) [MIM:125800]; also known as diabetes insipidus nephrogenic type 2. ANDI is caused by the inability of the renal collecting ducts to absorb water in response to arginine vasopressin. It is characterized by excessive water drinking (polydypsia), excessive urine excretion (polyuria), persistent hypotonic urine, and hypokalemia. Inheritance can be autosomal dominant or recessive.

Similarity:
Belongs to the MIP/aquaporin (TC 1.A.8) family.

SWISS:
P41181

Gene ID:
359

Database links:

Entrez Gene: 359 Human

Entrez Gene: 11827 Mouse

Entrez Gene: 25386 Rat

Omim: 107777 Human

SwissProt: P41181 Human

SwissProt: P51282 Mouse

SwissProt: P34080 Rat

Unigene: 130730 Human

Unigene: 20206 Mouse

Unigene: 90076 Rat

Picture

Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-AQP2 (Ser256)) Polyclonal Antibody, Unconjugated (SL12507R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Product Feedback Wall