KLH conjugated synthetic peptide derived from human ARIH2:41-140/493
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Sample: Pancreas (Mouse) Lysate at 40 ugPrimary: Anti-ARIH2 (SL12521R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 58 kDObserved band size: 58 kD
TRIAD1, also known as ARIH2 (ariadne homolog 2) or ARI2, is a 493 amino acid protein that contains one IBR-type zinc finger and two RING-type zinc fingers and belongs to the ariadne subfamily of RBR proteins. Localized to the nucleus, TRIAD1 interacts with UBE2L3 and is thought to act as an E3 ubiquitin-protein ligase, functioning to accept ubiquitin from E2 ubiquitin-conjugating enzymes and transfer the acquired ubiquitin residue to target substrates. TRIAD1 is subject to post-translational DNA damage-dependent phosphorylation, probably by ATM or ATR. The gene encoding TRIAD1 maps to human chromosome 3, which houses over 1,100 genes, including a chemokine receptor (CKR) gene cluster and a variety of human cancer-related gene loci.
Function: E3 ubiquitin-protein ligase mediating 'Lys-48'-and 'Lys-63'-linked polyubiquitination and subsequent proteasomal degradation of modified proteins. May play a role in myelopoiesis.
Subunit: Interacts (via RING-type 1) with UBE2L3. Interacts (via RING-type 2) with UBE2N. Interacts (via RING-type 2) with GFI1B. Interacts with GFI1; prevents its ubiquitination and proteasomal degradation.
Subcellular Location: Nucleus. Cytoplasm.
Tissue Specificity: Widely expressed with higher expression in granulocytes.
Post-translational modifications: Ubiquitinated. Ubiquitination promotes proteasomal degradation.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Similarity: Belongs to the RBR family. Ariadne subfamily.
Contains 1 IBR-type zinc finger.
Contains 2 RING-type zinc fingers.
