KLH conjugated synthetic peptide derived from human BOP1:201-300/746
Format:
Liquid
Storage instructions:
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration:
1mg/ml
Clonality:
Polyclonal
Isotype:
IgG
Applications:
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500(Paraffin sections need to do antigen repair)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host:
Rabbit
Product Overview:
Paraformaldehyde-fixed, paraffin embedded (Mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BOP1) Polyclonal Antibody, Unconjugated (SL12877R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Predominantly localized to the nucleolus, BOP1 (Block of proliferation 1 protein) is a 746 amino acid highly conserved non-ribosomal protein that is involved in ribosome biogenesis. Truncation of the amino terminus of BOP1 leads to cell growth arrest in the G1 phase and specific inhibition of 28S and 5.8S rRNA synthesis, as well as a deficit in the cytosolic 60S ribosomal subunit. This suggests that BOP1 is involved in the formation of mature rRNAs and in the biogenesis of the 60S ribosomal subunit. BOP1 physically interacts with pescadillo (a protein involved in cell proliferation) and enables efficient incorporation of pescadillo into the nucleolar preribosomal complexes, thereby affecting rRNA maturation and the cell cycle. The BOP1-pescadillo complex is also necessary for biogenesis of 60S ribosomal subunits. Deregulation of BOP1 may lead to colorectal tumorigenesis.
Function: Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome.
Subunit: Component of the PeBoW complex, composed of BOP1, PES1and WDR12. Within the PeBoW complex BOP1 interacts directly withPES1 and WDR12. The PeBoW complex also associates with the 66Spre-ribosome.
Subcellular Location: Nucleus
Similarity: Belongs to the WD repeat BOP1/ERB1 family.
Contains 7 WD repeats.
SWISS: Q14137
Gene ID: 23246
Database links:
Entrez Gene: 23246 Human
Entrez Gene: 12181 Mouse
Entrez Gene: 300050 Rat
Omim: 610596 Human
SwissProt: Q14137 Human
SwissProt: P97452 Mouse
SwissProt: Q562C2 Rat
Unigene:
645279 Human
Unigene: 4283 Mouse
Unigene: 101388 Rat
Picture
Paraformaldehyde-fixed, paraffin embedded (Mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BOP1) Polyclonal Antibody, Unconjugated (SL12877R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Product Feedback Wall
Specific References (1) | SL12877R has been referenced in 1 publications.
[IF=5.076] Jia Fangyuan. et al. BOP1 Knockdown Attenuates Neointimal Hyperplasia by Activating p53 and Inhibiting Nascent Protein Synthesis. Oxid Med Cell Longev. 2021;2021:5986260 WB,IF,IHC ; Human, Rat.