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Equilibrative NBMPR-sensitive nucleoside transporter; equilibrative nitrobenzylmercaptopurine riboside (NBMPR)-sensitive nucleoside transporter; Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter; Equilibrative nucleoside tr
Cat:
SL13077R
Species Reactivity：
Human,(predicted: Mouse,Rat,Cow,Horse,Sheep,Cynomolgus Monkey, Orangutan)
Immunogen：
KLH conjugated synthetic peptide derived from human ENT1/SLC29A1:201-300/456
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Paraformaldehyde-fixed, paraffin embedded (human Pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ENT1) Polyclonal Antibody, Unconjugated (SL13077R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Product PDFs
Datasheet:


Equilibrative nucleoside transporters (ENTs) regulate many physiological processes and are widely distributed in mammals, plants, yeasts, insects, nematodes and protozoans. They enable facilitated diffusion of hydrophilic nucleosides, such as adenosine and nucleoside analogs, across cell membranes. ENTs are required for uptake of antiviral and anticancer nucleoside drugs and influence a variety of physiological processes, such as neurotransmission and platelet aggregation, by regulating the amount of adenoside available to cell surface receptors. Equilibrative nucleoside transporter 1 (ENT1), also designated solute carrier family 29 (nucleoside transporters), member 1, belongs to the SLC29A transporter family and is a mammalian ENT isoform. ENT1, along with ENT3, mediates the majority of influx and efflux of nucleosides across the plasma membrane.

Function:
Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Inhibited by dipyridamole and dilazep (anticancer chemotherapeutics drugs).

Subcellular Location:
Basolateral cell membrane. Apical cell membrane. Predominantly localized in the basolateral membrane in polarised MDCK cells.

Tissue Specificity:
Expressed in heart, brain, mammary gland, erythrocytes and placenta, and also in fetal liver and spleen.

Post-translational modifications:
Glycosylated.

Similarity:
Belongs to the SLC29A transporter family.

SWISS:
Q91968

Gene ID:
2030

Database links:

Entrez Gene: 2030 Human

Entrez Gene: 63959 Mouse

Omim: 602193 Human

SwissProt: Q91968 Human

SwissProt: Q9JIM1 Mouse

Unigene: 25450 Human

Unigene: 29744 Mouse



Picture

Paraformaldehyde-fixed, paraffin embedded (human Pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ENT1) Polyclonal Antibody, Unconjugated (SL13077R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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