C21orf127; Chromosome 21 open reading frame 127; EC 2.1.1.-; HemK methyltransferase family member 2; HemK2; M.HsaHemK2P; MGC19995; MTQ2; N-6 adenine-specific DNA methyltransferase 1; N-6 adenine-specific DNA methyltransferase 1 (putative); N6-DNA-methyltr
Cat:
SL15453R
Species Reactivity：
Human,(predicted: Mouse,Rat,Horse,Sheep,)
Immunogen：
KLH conjugated synthetic peptide derived from human HEMK2:1-100/214
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Sample: A549 Cell (Human) Lysate at 30 ugPrimary: Anti-HEMK2 (SL15453R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 23kDObserved band size: 23kD
HEMK2 is a 214 amino acid protein that belongs to the methyltransferase superfamily and exists as multiple alternatively spliced isoforms. The gene encoding HEMK2 maps to human chromosome 21, which houses approximately 300 genes and comprises nearly 1.5% of the human genome. Chromosome 21-associated disorders include Alzheimer's disease, amyotrophic lateral sclerosis and, most notably, Down syndrome (also known as trisomy 21).
Function: Heterodimeric methyltransferase that catalyzes N5-methylation of ETF1 on 'Gln-185', using S-adenosyl L-methionine as methyl donor. ETF1 needs to be complexed to ERF3 in its GTP-bound form to be efficiently methylated. May play a role in the modulation of arsenic-induced toxicity. May be involved in the conversion of monomethylarsonous acid (3+) into the less toxic dimethylarsonic acid.
Subunit: Heterodimer with TRMT112.
Tissue Specificity: Widely expressed, with highest expression in parathyroid and pituitary glands, followed by adrenal gland and kidney, and lowest expression in leukocytes and mammary gland.
Similarity: Belongs to the eukaryotic/archaeal PrmSLCrelated family.
Sample: A549 Cell (Human) Lysate at 30 ug
Primary: Anti-HEMK2 (SL15453R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 23kD
Observed band size: 23kD
