Home > Product > Antibody > Rabbit Anti-HGS antibody
Hepatocyte growth factor regulated tyrosine kinase substrate; Hepatocyte growth factor-regulated tyrosine kinase substrate; HGF-regulated tyrosine kinase substrate; HGNC:4897; HGS; HGS_HUMAN; Hrs; Human growth factor regulated tyrosine kinase substrate; P
Cat:
SL15473R
Species Reactivity:
Rat,(predicted: Human,Mouse,Dog,Cow,Horse,Sheep,)
Immunogen:
KLH conjugated synthetic peptide derived from human HGS :301-400/777
Format:
Liquid
Storage instructions:
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration:
1mg/ml
Clonality:
Polyclonal
Isotype:
IgG
Applications:
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500(Paraffin sections need to do antigen repair)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host:
Rabbit
Product Overview:
Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HGS) Polyclonal Antibody, Unconjugated (SL15473R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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Product PDFs
Datasheet:


HGS is a regulates endosomal sorting and plays a critical role in the recycling and degradation of membrane receptors. The encoded protein sorts monoubiquitinated membrane proteins into the multivesicular body, targeting these proteins for lysosome-dependent degradation. [provided by RefSeq, Dec 2010].

Function:
Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the activin receptor complex. Involved in receptor recycling via its association with the CART complex, a multiprotein complex required for efficient transferrin receptor recycling but not for EGFR degradation.

Subunit:
Interacts with TRAK1. Interacts with TRAK2 (By similarity). Interacts with TRAK1. Component of the ESCRT-0 complex composed of STAM or STAM2 and HGS. Part of a complex at least composed of HSG, STAM2 (or probably STAM) and EPS15. Interacts with STAM. Interacts with STAM2. Interacts with EPS15; the interaction is direct, calcium-dependent and inhibited by SNAP25. Interacts with NF2; the interaction is direct. Interacts with ubiquitin; the interaction is direct. Interacts with VPS37C. Interacts with SMAD1, SMAD2 and SMAD3. Interacts with TSG101; the interaction mediates the association with the ESCRT-I complex. Interacts with SNAP25; the interaction is direct and decreases with addition of increasing concentrations of free calcium. Interacts with SNX1; the interaction is direct. Component of a 550 kDa membrane complex at least composed of HGS and SNX1 but excluding EGFR. Component of the CART complex, at least composed of ACTN4, HGS/HRS, MYO5B and TRIM3.

Subcellular Location:
Cytoplasm. Early endosome membrane; Peripheral membrane protein; Cytoplasmic side. Endosome, multivesicular body membrane; Peripheral membrane protein.

Tissue Specificity:
Ubiquitous expression in adult and fetal tissues with higher expression in testis and peripheral blood leukocytes.

Post-translational modifications:
Phosphorylated on Tyr-334. A minor site of phosphorylation on Tyr-329 is detected. Phosphorylation occurs in response to EGF, IL-2, GM-CSF and HGF.
Ubiquitinated by ITCH.

Similarity:
Contains 1 FYVE-type zinc finger.
Contains 1 UIM (ubiquitin-interacting motif) repeat.
Contains 1 VHS domain.

SWISS:
O14964

Gene ID:
9146

Database links:

Entrez Gene: 9146 Human

Entrez Gene: 15239 Mouse

Entrez Gene: 56084 Rat

Omim: 604375 Human

SwissProt: O14964 Human

SwissProt: Q99LI8 Mouse

SwissProt: Q9JJ50 Rat

Unigene: 514590 Human

Unigene: 7919 Mouse

Unigene: 21 Rat



Picture

Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HGS) Polyclonal Antibody, Unconjugated (SL15473R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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