Sample:
Lane 1: Human Hela cell (UV) Lysates
Lane 2: Human A431 cell (UV) Lysates
Lane 3: Human U251 cell (UV) Lysates
Lane 4: Human 293T cell (TPA) Lysates
Primary: Anti-Phospho-Smad1 (SL10380R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51kDa
Observed band size: 63kDa
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (mono methyl K4)) Polyclonal Antibody, Unconjugated (SL2814R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.