CD 312; CD 312 antigen; CD312; CD312 antigen; Egf like module containing mucin like hormone receptor like 2; Egf like module containing mucin like hormone receptor like sequence 2; EGF like module EMR2; EGF-like module receptor 2; EGF-like module-containi
Cat:
SL13634R
Species Reactivity：
Human,
Immunogen：
KLH conjugated synthetic peptide derived from human EMR2:451-550/823<Extracellular>
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
WB=1:500-2000ELISA=1:5000-10000not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Sample: TM4(Mouse) Cell Lysate at 30 ugMCF-7(Human) Cell Lysate at 30 ugPrimary: Anti-EMR2 (SL13634R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 88 kDObserved band size: 128 kD
The epidermal growth factor (EGF) family constitutes a group of class B, G protein-coupled receptors, which includes CD97 and EMR2. EMR2 is a member of the EGF-TM7 receptor subfamily. EGF-TM7 receptors are a family of class B, seven-span transmembrane (TM7) receptors predominantly expressed by cells of the immune system. Within the TM7 superfamily, the molecular structure and ligand-binding properties of EGF-TM7 receptors are unique. Derived from the processing of a single polypeptide, they are expressed at the cell surface as heterodimers consisting of a large extracellular region associated with a TM7 moiety. Through a variable number of N-terminal EGF-like domains, EGF-TM7 receptors interact with cellular ligands such as CD55 and chondroitin sulfate. EMR2 is a heptahelical molecule predominantly expressed on cells of the immune system such as leukocytes. EMR2 is proteo-lytically cleaved into two separate subunits: a seven-transmembrane subunit, and an extracellular Alpha subunit.
Function: Receptor probably involved in cell attachment.
Subcellular Location: Cell membrane.
Tissue Specificity: Expression is restricted to myeloid cells. Highest expression was found in peripheral blood leukocytes, followed by spleen and lymph nodes, with intermediate to low levels in thymus, bone marrow, fetal liver, placenta, and lung, and no expression in heart, brain, skeletal muscle, kidney, or pancreas. Expression is also detected in monocyte/macrophage and Jurkat cell lines but not in other cell lines tested.
Post-translational modifications: modificationsAutoproteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane beta subunit.
Similarity: Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.
Contains 5 EGF-like domains. Contains 1 GPS domain.
Sample:
TM4(Mouse) Cell Lysate at 30 ug
MCF-7(Human) Cell Lysate at 30 ug
Primary: Anti-EMR2 (SL13634R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 88 kD
Observed band size: 128 kD
