Rabbit Anti-GADD34 antibody

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Growth arrest and DNA damage-inducible 34; Apoptosis associated protein; GADD 34; Growth arrest and DNA damage inducible 34; Growth arrest and DNA-damage-inducible 34; PPP1R15A; Protein phosphatase 1 regulatory (inhibitor) subunit 15A; Protein phosphatase

Cat:

SL10287R

Species Reactivity：

Human,(predicted: Mouse,Rat,)

Immunogen：

KLH conjugated synthetic peptide derived from human GADD34:401-500/674

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1μg/TestICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Blank control(blue): 293T Cells(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti-GADD34/AF647 Conjugated antibody (SL10287R-AF647), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The induction of this gene by ionizing radiation occurs in certain cell lines regardless of p53 status, and its protein response is correlated with apoptosis following ionizing radiation. [provided by RefSeq, Jul 2008]

Function:
Recruits the serine/threonine-protein phosphatase PP1 to dephosphorylate the translation initiation factor eIF-2A/EIF2S1, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress.

Subunit:
Interacts with PCNA. Interacts with LYN and MLL. Interacts with PP1, PPP1R1A and SMARCB1. Interacts with SMAD7. Interacts with BAG1.

Subcellular Location:
Endoplasmic reticulum.

Post-translational modifications:
Phosphorylated on tyrosine by LYN; which impairs its antiproliferative activity.

Similarity:
Belongs to the PPP1R15 family.

SWISS:
O75807

Gene ID:
23645

Database links:

Entrez Gene: 514688 Cow

Entrez Gene: 23645 Human

Entrez Gene: 17872 Mouse

Entrez Gene: 171071 Rat

Omim: 611048 Human

SwissProt: Q2KI51 Cow

SwissProt: O75807 Human

SwissProt: P17564 Mouse

SwissProt: Q6IN02 Rat

Unigene: 631593 Human

Unigene: 4048 Mouse

Unigene: 2232 Rat

Picture

Blank control(blue): 293T Cells(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice).
Primary Antibody: Rabbit Anti-GADD34/AF647 Conjugated antibody (SL10287R-AF647), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions.

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