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Home > Product > Antibody > Rabbit Anti-EDDM3A antibody
EP3A; Epididymal protein 3A; Epididymal secretory protein E3 alpha; Epididymis specific 3 alpha; FAM12A; EP3A_HUMAN; Family with sequence similarity 12 member A; HE3 ALPHA; HE3A; HE3ALPHA; Human epididymis specific 3 alpha; Human epididymis spec
Cat:
SL14499R
Species Reactivity：
Human,
Immunogen：
KLH conjugated synthetic peptide derived from human EDDM3A:31-130/147
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Paraformaldehyde-fixed, paraffin embedded (human prostate); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EDDM3A) Polyclonal Antibody, Unconjugated (SL14499R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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Unit:
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Product PDFs
Datasheet:


Testicular sperm are morphologically differentiated but are not progressively motile nor able to fertilize an egg. Post-testicular maturation requires exposure of spermatozoa to the microenvironment of the epididymal lumen. Spermatozoa undergo extensive changes in the epididymis, including enzymatic modifications, loss of pre-existing components and addition of new glycoproteins from epididymal secretions. These modifying proteins and enzymes are synthesized by epithelial cells lining the epididymal duct and secreted apically into the lumen, where they come into contact with, and may be absorbed onto, the sperm membranes. The proteins encoded by the genes in this cluster are synthesized and secreted by epididymal epithelial cells. [provided by RefSeq, Jul 2008]

Function:
Possible function in sperm maturation.

Subcellular Location:
Secreted

Tissue Specificity:
Epididymis, with predominant expression in the corpus region. Moderately expressed in the vas deferens; only low levels are detectable in the caput and cauda regions.

SWISS:
Q14507

Gene ID:
10876

Database links:

Entrez Gene: 10876 Human

Omim: 61316 Human

SwissProt: Q14507 Human

Unigene: 304757 Human



Picture

Paraformaldehyde-fixed, paraffin embedded (human prostate); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EDDM3A) Polyclonal Antibody, Unconjugated (SL14499R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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