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Home > Product > Antibody > Rabbit Anti-Hrk antibody
Activator of apoptosis harakiri; Activator of apoptosis Hrk; BCL2 interacting protein; BH3 interacting domain protein 3; BID3; DP5; Harakiri BCL2 interacting protein; Human brain 3UTR of mRNA for neuronal death protein partial sequence; Neuronal death pro
Cat:
SL10302R
Species Reactivity：
Rat,(predicted: Human,Mouse,Dog,Cow,)
Immunogen：
KLH conjugated synthetic peptide derived from human Activator of apoptosis harakiri:1-50/91
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Hrk) Polyclonal Antibody, Unconjugated (SL10302R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Product PDFs
Datasheet:


This gene encodes a member of the BCL-2 protein family. Members of this family are involved in activating or inhibiting apoptosis. The encoded protein localizes to intracellular membranes. This protein promotes apoptosis by interacting with the apoptotic inhibitors BCL-2 and BCL-X(L) via its SH3 domain. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Oct 2012]

Function:
Promotes apoptosis.

Subunit:
Interacts with BCL2 and BCL2L1. Interacts with C1QBP.

Subcellular Location:
Membrane; Single-pass membrane protein. Mitochondrion.

SWISS:
O00198

Gene ID:
8739

Database links:

Entrez Gene: 8739 Human

Entrez Gene: 12123 Mouse

Omim: 603447 Human

SwissProt: O00198 Human

SwissProt: P62816 Mouse

SwissProt: P70678 Mouse

Unigene: 87247 Human



Picture

Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Hrk) Polyclonal Antibody, Unconjugated (SL10302R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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