FAM175B (ABRO1) is a 415 amino acid protein, sharing 39% identity to ABRA1 in the N terminal two thirds of the protein (amino acids 1 to 260), including a coiled-coil domain and a region called the ABR domain. However, ABRO1 lacks the phosphorylated ser-X-X-phe motif of ABRA1 and, unlike ABRA1, does not bind to BRCA1.
Function:
Component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin. May act as a central scaffold protein that assembles the various components of the BRISC complex.
Subunit:
Component of the BRISC complex, at least composed of the FAM175B/ABRO1, BRCC3/BRCC36, BRE/BRCC45 and BABAM1/NBA1. Does not interact with BRCA1. Binds polyubiquitin.
Similarity:
Belongs to the FAM175 family. Abro1 subfamily.
SWISS:
Q15018
Gene ID:
23172
Database links:
Entrez Gene: 23172 Human
Entrez Gene: 109359 Mouse
Entrez Gene: 293570 Rat
Omim: 611144 Human
SwissProt: Q15018 Human
SwissProt: Q3TCJ1 Mouse
| Picture |
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FAM175B) Polyclonal Antibody, Unconjugated (SL14763R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
|
|
|
