Rabbit Anti-OTUD5/DUBA antibody

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Deubiquinating enzyme A; Deubiquitinating enzyme A; DKFZp761A052; DUBA; MGC104871; OTTHUMP00000025821; OTU domain containing 5; OTU domain containing protein 5; OTU domain-containing protein 5; OTUD 5; otud5; OTUD5_HUMAN.

Cat:

SL17566R

Species Reactivity：

Human,(predicted: Mouse,Rat,Chicken,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human OTUD5/DUBA :501-571/571

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Tissue/cell: human cervical cancer; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-OTUD5 Polyclonal Antibody, Unconjugated(SL17566R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a member of the OTU (ovarian tumor) domain-containing cysteine protease superfamily. The OTU domain confers deubiquitinase activity and the encoded protein has been shown to suppress the type I interferon-dependent innate immune response by cleaving the polyubiquitin chain from an essential type I interferon adaptor protein. Cleavage results in disassociation of the adaptor protein from a downstream signaling complex and disruption of the type I interferon signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Oct 2008]

Function:
Negative regulator of innate immune system. Has deubiquitinating activity that is directed towards 'Lys-63'-linked polyubiquitin chains. Acts via TRAF3 deubiquitination and subsequent suppression of type I interferon (IFN) production.

Subunit:
Interacts with TRAF3.

Tissue Specificity:
Expressed in various tissues, including the liver and placenta, as well as in peripheral blood leukocytes.

Post-translational modifications:
Phosphorylation at Ser-177 is required for deubiquitinating activity.

Similarity:
Belongs to the peptidase C85 family.
Contains 1 OTU domain.

SWISS:
Q96G74

Gene ID:
55593

Database links:

Entrez Gene: 55593 Human

Entrez Gene: 54644 Mouse

Entrez Gene: 363452 Rat

Omim: 300713 Human

SwissProt: Q96G74 Human

SwissProt: Q3U2S4 Mouse

SwissProt: Q2YDU3 Rat

Unigene: 496098 Human

Unigene: 142827 Mouse

Unigene: 97794 Rat

Picture

Tissue/cell: human cervical cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-OTUD5 Polyclonal Antibody, Unconjugated(SL17566R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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