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Home > Product > Antibody > Rabbit Anti-SPTBN3 antibody
Spectrin beta chain, brain 3; Beta-IV spectrin; brain 3; KIAA1642; non-erythroid beta chain 3; QV; Spectrin; Spectrin beta chain; spectrin, beta, non-erythrocytic 4; spectrin, non-erythroid beta chain 3; SPNB4; SPTBN3; SPTBN4; SPTN4_HUMAN.
Cat:
SL17649R
Species Reactivity：
Mouse,(predicted: Human,Rat,Cow,Horse,Rabbit,Sheep,)
Immunogen：
KLH conjugated synthetic peptide derived from human SPTBN3:2401-2500/2564
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPTBN3) Polyclonal Antibody, Unconjugated (SL17649R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Product PDFs
Datasheet:


Spectrin, an actin binding protein that is a major component of the cytoskeletal superstructure of the erythrocyte plasma membrane, is essential in determining the properties of the membrane including its shape and deformability. Spectrins function as membrane organizers and stabilizers, composed of nonhomologous Alpha and beta chains, which aggregate side-to-side in an antiparallel fashion to form dimers, tetramers, and higher polymers. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function. Spectrin beta IV is a non-erythrocytic member of the beta-spectrin family. It is expressed in brain and pancreatic islets and localizes to the nuclear matrix, cytoplasmic vesicles and PML nuclear bodies. Spectrin beta IV is a 2,564 amino acid protein with four isoforms due to alternative splicing events.

Subcellular Location:
Cytoplasm > cytoskeleton. Cytoplasm > cell cortex.

Tissue Specificity:
Abundantly expressed in brain and pancreatic islets.

Similarity:
Belongs to the spectrin family.
Contains 2 CH (calponin-homology) domains.
Contains 1 PH domain.
Contains 18 spectrin repeats.

SWISS:
Q9H254

Gene ID:
57731

Database links:

Entrez Gene: 57731 Human

Omim: 606214 Human

SwissProt: Q9H254 Human

Unigene: 32706 Human



Picture

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPTBN3) Polyclonal Antibody, Unconjugated (SL17649R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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