SPESP1 is a 399 amino acid protein belonging to the SPESP1 family. Localizing to cytoplasmic vesicle, secretory vesicle, and acrosome, SPESP1 is highly expressed in testis, with lower levels found in placenta and fetal lung. SPESP1 establishes an equatorial segment subcompartment early in sperm development and is required for proper sperm-egg fusion. Disruption of SPESP1 leads to abnormal distribution of sperm proteins resulting in a detached membrane from the equatorial segment and less fertile sperm. SPESP1 may interact with IZUMO1 and MN9 antigen and contains an N-glycosylation site as well as several cAMP-dependent kinase, protein kinase C, and casein kinase II consensus phosphorylation sites.
Function:
The encoded protein is a human alloantigen involved in sperm-egg binding and fusion. [provided by RefSeq, Apr 2010]
Subcellular Location:
Cytoplasmic vesicle > secretory vesicle > acrosome. Small proacrosomal granules (during the Golgi phase), enlarged acrosomal vesicles (during the cap phase), acrosome (during the elongating phase), equatorial segment of the acrosome.
Tissue Specificity:
Highly expressed in testis, where it is localized in the acrosome of postmeiotic stages of spermiogenesis (round and elongating spermatids and in ejaculated spermatozoa) (at protein level). Poorly expressed in placenta and fetal lung.
Similarity:
Belongs to the SPESP1 family.
SWISS:
Q6UW49
Gene ID:
246777
Database links:
Entrez Gene: 246777 Human
Omim: 609399 Human
SwissProt: Q6UW49 Human
Unigene: 657932 Human
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Paraformaldehyde-fixed, paraffin embedded (human liver cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPESP1) Polyclonal Antibody, Unconjugated (SL17655R) at 1:800 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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