Rabbit Anti-LIPC antibody | Sunlong Medical

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HDLCQ12; Hepatic lipase; Hepatic triacylglycerol lipase; HL; HTGL; Lipase member C; lipC; LIPC_HUMAN; LIPH.

Cat:

SL18288R

Species Reactivity：

Human,Mouse,Rat,(predicted: Dog,Cow,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human LIPC:301-400/499

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Mouse Liver tissue lysates Lane 2: Mouse Kidney tissue lysates Lane 3: Rat Liver tissue lysates Lane 4: Rat Kidney tissue lysates Primary: Anti-LIPC (SL18288R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 53 kDObserved band size: 53 kDTissue/cell: human liver carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-LIPC Polyclonal Antibody, Unconjugated(SL18288R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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LIPC encodes hepatic triglyceride lipase, which is expressed in liver. LIPC has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake. [provided by RefSeq, Jul 2008]

Function:
Hepatic lipase has the capacity to catalyze hydrolysis of phospholipids, mono-, di-, and triglycerides, and acyl-CoA thioesters. It is an important enzyme in HDL metabolism. Hepatic lipase binds heparin.

Subcellular Location:
Secreted.

DISEASE:
Defects in LIPC are the cause of hepatic lipase deficiency (HL deficiency) [MIM:151670].

Similarity:
Belongs to the AB hydrolase superfamily. Lipase family.
Contains 1 PLAT domain.

SWISS:
P11150

Gene ID:
3990

Database links:

Entrez Gene: 3990 Human

Omim: 151670 Human

SwissProt: P11150 Human

Unigene: 654472 Human

Picture

Tissue/cell: human liver carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-LIPC Polyclonal Antibody, Unconjugated(SL18288R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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