The protein encoded by this gene was first identified by its binding to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. This protein localizes to microtubules, especially the growing ends, in interphase cells. During mitosis, the protein is associated with the centrosomes and spindle microtubules. The protein also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. Because of these associations, it is thought that this protein is involved in the regulation of microtubule structures and chromosome stability. This gene is a member of the RP/EB family. [provided by RefSeq, Jul 2008]
Function:
May be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration.
Subcellular Location:
Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Associated with the microtubule network at the growing distal tip of microtubules. Also enriched at the centrosome.
Tissue Specificity:
Ubiquitously expressed.
Similarity:
Belongs to the MAPRE family.
Contains 1 CH (calponin-homology) domain.
Contains 1 EB1 SLCterminal domain.
SWISS:
Q15691
Gene ID:
22919
Database links:
Entrez Gene: 22919 Human
Entrez Gene: 419288 Chicken
Entrez Gene: 13589 Mouse
Entrez Gene: 10017646 Orangutan
Entrez Gene: 114764 Rat
Omim: 603108 Human
SwissProt: Q5ZLC7 Chicken
SwissProt: Q15691 Human
SwissProt: Q61166 Mouse
SwissProt: Q5R7Z5 Orangutan
SwissProt: Q6V291 Quail
SwissProt: Q66HR2 Rat
Unigene: 472437 Human
Unigene: 143877 Mouse
Unigene: 7652 Rat
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Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-MAPRE1 Polyclonal Antibody, Unconjugated(SL18675R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
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