3 N(4) ethenocytosine glycosylase;
G/5 fluorouracil mismatch glycosylase with biphasic kinetics;
G/T mismatch glycosylase;
G/U mismatch glycosylase;
MBD 4;
MBD4;
MBD4_HUMAN;
MED 1;
MED1;
Methyl CpG binding domain protein 4;
Methyl CpG binding en
Cat:
SL18704R
Species Reactivity：
Human,(predicted: Mouse,Rat,Pig,Cow,Sheep,)
Immunogen：
KLH conjugated synthetic peptide derived from human MBD4:361-460/580
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Sample: A549(Human) Cell Lysate at 30 ugPrimary: Anti-MBD4 (SL18704R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 66kDObserved band size: 66 kD
The protein encoded by this gene is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD). These proteins are capable of binding specifically to methylated DNA, and some members can also repress transcription from methylated gene promoters. This protein contains an MBD domain at the N-terminus that functions both in binding to methylated DNA and in protein interactions and a SLCterminal mismatch-specific glycosylase domain that is involved in DNA repair. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013]
Function: Mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein.
Sample:
A549(Human) Cell Lysate at 30 ug
Primary: Anti-MBD4 (SL18704R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 66kD
Observed band size: 66 kD
