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Home > Product > Antibody > Rabbit Anti-NDFIP2 antibody
Ndfip2; NEDD4 family interacting protein 2; NEDD4 family-interacting protein 2; NEDD4 WW domain binding protein 5A; NEDD4 WW domain-binding protein 5A; NFIP2_HUMAN; Putative MAPK activating protein PM04/PM05/PM06/PM07; Putative MAPK-activating prot
Cat:
SL19059R
Species Reactivity：
Human,(predicted: Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep,)
Immunogen：
KLH conjugated synthetic peptide derived from human NDFIP2:261-336/336<Extracellular>
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Sample: Siha Cell (Human) Lysate at 40 ugPrimary: Anti- NDFIP2 (SL19059R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 36 kDObserved band size: 34 kD
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Product PDFs
Datasheet:


NDFIP2 is a 336 amino acid protein that localizes to the membrane of both the endosome and the golgi apparatus. Expressed in kidney, heart, brain, lung, liver, placenta and skeletal muscle, Ndfip2 interacts with NEDD4 and NEDD4-L and is thought to be involved in endocytosis and in the NFⅹB and MAPK signaling pathways. Additionally, Ndfip2 may function as an adaptor protein that may recruit NEDD4 ubiquitin-protein ligases to protein trafficking machinery in the golgi. Ndfip2 is activated by T cells and may be ubiquitinated by NEDD4 or NEDD4-L, an event that does not affect Ndfip2 function. The gene encoding Ndfip2 maps to human chromosome 13, which houses over 400 genes, such as BRCA2 and RB1, and comprises nearly 4% of the human genome.

Function:
Activates HECT domain-containing E3 ubiquitin-protein ligases, including ITCH, NEDD4, NEDD4L, SMURF2, WWP1 and WWP2, and consequently modulates the stability of their targets. As a result, may control many cellular processes. Recruits ITCH, NEDD4 and SMURF2 to endosomal membranes. May modulate EGFR signaling.

Subunit:
Forms heterodimers with NDFIP1. Interacts with HECT domain-containing E3 ubiquitin-protein ligases, including NEDD4. Interacts with NEDD4L By similarity. Interacts with PTEN. When phosphorylated at Tyr-167, interacts with SRC and LYN SH2 domain. May thus act as a scaffold that recruits SRC to NDFIP1, enhancing NDFIP1 phosphorylation. Interacts with SLC11A2/DMT1. May interact with phosphorylated EGFR.

Subcellular Location:
Endosome membrane. Golgi apparatus membrane. Endosome > multivesicular body membrane.

Tissue Specificity:
Expressed in brain, lung, heart, skeletal muscle, kidney, liver and placenta.

Post-translational modifications:
Ubiquitinated by NEDD4 and ITCH. Also ubiquitinated by NEDD4L. Ubiquitination by NEDD4 or NEDD4L does not affect turnover.
Undergoes transient tyrosine-phosphorylation following EGF stimulation, most probably catalyzed by SRC. Also phosphorylated by LYN and FYN.

SWISS:
Q9NV92

Gene ID:
5922

Database links:

Entrez Gene: 76273 Mouse

Entrez Gene: 361089 Rat

SwissProt: Q91ZP6 Mouse

SwissProt: F1M5D2 Rat

Unigene: 290669 Mouse

Unigene: 387487 Mouse



Picture

Sample: Siha Cell (Human) Lysate at 40 ug
Primary: Anti- NDFIP2 (SL19059R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 34 kD
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