This gene encodes a subunit of tRNA-guanine transglycosylase. tRNA-guanine transglycosylase is a heterodimeric enzyme complex that plays a critical role in tRNA modification by synthesizing the 7-deazaguanosine queuosine, which is found in tRNAs that code for asparagine, aspartic acid, histidine, and tyrosine. The encoded protein may play a role in the queuosine 5'-monophosphate salvage pathway. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012]
Function:
Interacts with QTRT1 to form an active queuine tRNA-ribosyltransferase. This enzyme exchanges queuine for the guanine at the wobble position of tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), thereby forming the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).
Subunit:
Interacts with QTRT1.
Subcellular Location:
Cytoplasm. Mitochondrion. May associate with the mitochondrion outer membrane.
Similarity:
Belongs to the queuine tRNA-ribosyltransferase family. QTRTD1 subfamily.
SWISS:
Q9H974
Gene ID:
79691
Database links:
Entrez Gene: 508374 Cow
Entrez Gene: 79691 Human
Entrez Gene: 106248 Mouse
Entrez Gene: 288364 Rat
SwissProt: Q9H974 Human
SwissProt: B8ZXI1 Mouse
Unigene: 477162 Human
Unigene: 275354 Mouse
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (QTRTD1) Polyclonal Antibody, Unconjugated (SL19700R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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