Rabbit Anti-HGF beta antibody

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HGF-beta; HGFB; F TCF; Hepatocyte growth factor; Fibroblast derived tumor cytotoxic factor; Hepatocyte growth factor precursor; Hepatopoietin A; HPTA; Lung fibroblast derived mitogen; Scatter factor; SF; hepatocyte growth factor beta-chain; Hepatocyte gro

Cat:

SL20688R

Species Reactivity：

Human,Mouse,(predicted: Rat,Dog,Cow,Horse,)

Immunogen：

KLH conjugated synthetic peptide derived from human HGF beta:501-600/723

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Liver (Mouse) Lysate at 40 ugPrimary: Anti-HGF beta (SL20688R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 26/80 kDObserved band size: 26/80 kDBlank control:U937. Primary Antibody (green line): Rabbit Anti-HGF beta antibody (SL20688R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647Dilution: 1μg /test. ProtocolThe cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the lasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008].

Function:
HGF is a potent mitogen for mature parenchymal hepatocyte cells, seems to be an hepatotrophic factor, and acts as growth factor for a broad spectrum of tissues and cell types. It has no detectable protease activity.

Subunit:
Dimer of an alpha chain and a beta chain linked by a disulfide bond.

DISEASE:
Defects in HGF are the cause of deafness autosomal recessive type 39 (DFNB39) [MIM:608265]. A form of profound prelingual sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.

Similarity:
Belongs to the peptidase S1 family. Plasminogen subfamily.
Contains 4 kringle domains.
Contains 1 PAN domain.

SWISS:
P14210

Gene ID:
3082

Database links:

Entrez Gene: 3082 Human

Entrez Gene: 282879 Cow

Entrez Gene: 15234 Mouse

Entrez Gene: 24446 Rat

Omim: 14489 Human

SwissProt: Q9SH09 Cat

SwissProt: Q76BS1 Cow

SwissProt: Q867B7 Dog

SwissProt: P14210 Human

SwissProt: Q08048 Mouse

SwissProt: P17945 Rat

Unigene: 396530 Human

Unigene: 267078 Mouse

Unigene: 10468 Rat

Picture

Blank control:U937.
Primary Antibody (green line): Rabbit Anti-HGF beta antibody (SL20688R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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