Home > Product > Antibody > Rabbit Anti-DUSP21 antibody
Dual specificity phosphatase 21; Dual specificity protein phosphatase 21; DUS21_MOUSE; DUSP21; LMW-DSP21; LMWDSP21; Low molecular weight dual specificity phosphatase 21.
Cat:
SL20849R
Species Reactivity:
Rat,(predicted: Mouse,)
Immunogen:
KLH conjugated synthetic peptide derived from mouse DUSP21:41-140/190
Format:
Liquid
Storage instructions:
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration:
1mg/ml
Clonality:
Polyclonal
Isotype:
IgG
Applications:
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500(Paraffin sections need to do antigen repair)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host:
Rabbit
Product Overview:
Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DUSP21) Polyclonal Antibody, Unconjugated (SL20849R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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Unit:
Price: $
Product PDFs
Datasheet:


This gene encodes a member of the dual specificity phosphatase family, specifically the low molecular weight dual specificity phosphatase family. The encoded protein localizes to both the cytoplasm and the nucleus and functions to remove phosphate groups from phosphotyrosine and phosphothreonine residues.[provided by RefSeq, Mar 2009]

Function:
Can dephosphorylate single and diphosphorylated synthetic MAPK peptides, with preference for the phosphotyrosine and diphosphorylated forms over phosphothreonine.

Subcellular Location:
Cytoplasm. Nucleus.

Tissue Specificity:
Expressed in testis.

Similarity:
Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
Contains 1 tyrosine-protein phosphatase domain.

SWISS:
Q9D9D8

Gene ID:
73547

Database links:

Entrez Gene: 73547 Mouse

SwissProt: Q9D9D8 Mouse



Picture

Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DUSP21) Polyclonal Antibody, Unconjugated (SL20849R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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