Recombinant human IL-6 corresponding to 30-212/212aa (C-6x His-Tag)
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/1ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
WB=1:500-2000ELISA=1:5000-10000not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Sample: Lane 1: Recombinant human IL-6 protein, SLCHis (SL10807P) Lane 2: Recombinant human IL-6 protein, SLCHis (SL41177P) Primary: Anti-IL-6 (SL10807R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 23 kDaObserved band size: 16 kDaSample: HUVEC(Human) Cell Lysate at 30 ugPrimary: Anti-IL-6 (SL10807R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 23 kDObserved band size: 23 kD
This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. [provided by RefSeq, Jun 2011].
Function: Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
Subcellular Location: Secreted.
Post-translational modifications: N- and O-glycosylated.
DISEASE: Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis. Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HISLVinfected men.
Similarity: Belongs to the IL-6 superfamily.
SWISS: P05231
Gene ID: 3569
Database links:
Entrez Gene: 3569 Human
Entrez Gene: 16193 Mouse
Entrez Gene: 24498 Rat
Omim: 147620 Human
SwissProt: P05231 Human
SwissProt: P08505 Mouse
SwissProt: P20607 Rat
Unigene: 654458 Human
Unigene: 1019 Mouse
Unigene: 9873 Rat
Picture
Sample:
Lane 1: Recombinant human IL-6 protein, SLCHis (SL10807P)
Lane 2: Recombinant human IL-6 protein, SLCHis (SL41177P)
Primary: Anti-IL-6 (SL10807R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 23 kDa
Observed band size: 16 kDa
Sample:
HUVEC(Human) Cell Lysate at 30 ug
Primary: Anti-IL-6 (SL10807R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 23 kD
Observed band size: 23 kD
Product Feedback Wall
Specific References (1) | SL10807R has been referenced in 1 publications.
[IF=2.376] Lifei Li. et al. GnRH agonist treatment regulates IL-6 and IL-11 expression in endometrial stromal cells for patients with HRT regiment in frozen embryo transfer cycles. Reprod Biol. 2022 Jun;22:100608 WB ; Human.
