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Home > Product > Antibody > Rabbit Anti-CD2AP antibody
CD2-associated protein; Adapter protein CMS; AL024079; Cas ligand with multiple SH3 domains; C78928; Cd2ap; CMS; Mesenchyme to epithelium transition protein with SH3 domains 1; METS 1; Mets1; CD2AP_HUMAN.
Cat:
SL22213R
Species Reactivity：
Rat,(predicted: Human,Mouse,Pig,Cow,Horse,Rabbit,Sheep,)
Immunogen：
KLH conjugated synthetic peptide derived from human CD2AP:441-540/639
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
IHC-P=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD2AP) Polyclonal Antibody, Unconjugated (SL22213R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Datasheet:


This gene encodes a scaffolding molecule that regulates the actin cytoskeleton. The protein directly interacts with filamentous actin and a variety of cell membrane proteins through multiple actin binding sites, SH3 domains, and a proline-rich region containing binding sites for SH3 domains. The cytoplasmic protein localizes to membrane ruffles, lipid rafts, and the leading edges of cells. It is implicated in dynamic actin remodeling and membrane trafficking that occurs during receptor endocytosis and cytokinesis. Haploinsufficiency of this gene is implicated in susceptibility to glomerular disease. [provided by RefSeq, Jul 2008].

Function:
Seems to act as an adapter protein between membrane proteins and the actin cytoskeleton. May play a role in receptor clustering and cytoskeletal polarity in the junction between T-cell and antigen-presenting cell. May anchor the podocyte slit diaphragm to the actin cytoskeleton in renal glomerolus. Also required for cytokinesis.

Subunit:
Self-associates. Homodimer (Potential). Interacts with F-actin, PKD2, NPHS1 and NPHS2. Interacts with WTIP. Interacts with DDN; interaction is direct. Interacts (via SH3 2 domain) with CBL (via phosphorylated SLCterminus). Interacts with BCAR1/p130Cas (via SH3 domain). Interacts with MVB12A and ARHGAP17. Interacts with ANLN, CD2 and CBLB. Interacts with PDCD6IP and TSG101. Interacts with RIN3.

Subcellular Location:
Cytoplasm, cytoskeleton. Cell projection, ruffle. Note=Colocalizes with F-actin and BCAR1/p130Cas in membrane ruffles. Located at podocyte slit diaphragm between podocyte foot processes. During late anaphase and telophase, concentrates in the vicinity of the midzone microtubules and in the midbody in late telophase.

Tissue Specificity:
Widely expressed in fetal and adult tissues.

Post-translational modifications:
Phosphorylated on tyrosine residues; probably by c-Abl, Fyn and c-Src.

DISEASE:
Focal segmental glomerulosclerosis 3 (FSGS3) [MIM:607832]: A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and progressive decline in renal function. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.

Similarity:
Contains 3 SH3 domains.

SWISS:
Q9Y5K6

Gene ID:
2727

Database links:
Entrez Gene: 2727 Human

Entrez Gene: 12488 Mouse

Entrez Gene: 316258 Rat

Omim: 604241 Human

SwissProt: Q9Y5K6 Human

SwissProt: Q9JLQ0 Mouse

Unigene: 485518 Human

Unigene: 218637 Mouse

Unigene: 212220 Rat



CD2AP可能作为细胞裂孔隔膜分子与细胞骨架的连接蛋白，在细胞分化即增殖的过程中发挥重要作用。 目前主用用于肾脏功能方面的研究，D2AP不仅参与T细胞的活化，而且对肾脏功能起着至关重要的作用，其异常表达可能是引起人类肾脏疾病的诱因之一。 Picture

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD2AP) Polyclonal Antibody, Unconjugated (SL22213R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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