RECK is a cysteine rich, extracellular protein with protease inhibitor like domains whose expression is suppressed strongly in many tumors and cells transformed by various kinds of oncogenes. In normal cells, this membrane anchored glycoprotein may serve as a negative regulator for matrix metalloproteinase 9, a key enzyme involved in tumor invasion and metastasis.
Function:
Negatively regulates matrix metalloproteinase-9 (MMP-9) by suppressing MMP-9 secretion and by direct inhibition of its enzymatic activity. RECK down-regulation by oncogenic signals may facilitate tumor invasion and metastasis. Appears to also regulate MMP-2 and MT1-MMP, which are involved in cancer progression.
Subunit:
Interacts with MMP-9.
Subcellular Location:
Cell membrane; Lipid-anchor, GPI-anchor.
Tissue Specificity:
Expressed in various tissues and untransformed cells. It is undetectable in tumor-derived cell lines and oncogenically transformed cells.
Post-translational modifications:
N-glycosylated.
Similarity:
Contains 3 Kazal-like domains.
SWISS:
O95196
Gene ID:
313488
Database links:
Entrez Gene: 313488 Human
Entrez Gene: 8434 Human
Omim: 605227 Human
SwissProt: O95196 Human
SwissProt: Q5W0K6 Human
Unigene: 388918 Human
Unigene: 728961 Human
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Sample:
Lane 1: Mouse Kidney tissue lysates
Lane 2: Mouse NIH/3T3 cell lysates
Lane 3: Human Huvec cell lysates
Lane 4: Human U87MG cell lysates
Primary: Anti-RECK (SL2901R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 107 kDa
Observed band size: 120 kDa
Blank control:U937.
Primary Antibody (green line): Rabbit Anti-RECK antibody (SL2901R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells wereincubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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