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Home > Product > Antibody > Rabbit Anti-Seladin 1 antibody
Seladin1; Seladin-1; DHCR24; 24 dehydrocholesterol reductase; 3-beta-hydroxysterol delta-24-reductase; DCE; Desmosterol to cholesterol enzyme; Diminuto dwarf1 homolog; SELADIN1; Selective AD indicator 1; Selective Alzheimer's Disease Indicator 1.
Cat:
SL5390R
Species Reactivity：
Mouse,Rat,(predicted: Human,Dog,Pig,Cow,Horse,Rabbit,)
Immunogen：
KLH conjugated synthetic peptide derived from human Seladin 1:101-200/516
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Seladin 1) Polyclonal Antibody, Unconjugated (SL5390R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Product PDFs
Datasheet:


Seladin 1 is highly expressed in brain, adrenal tissues and adrenal tumours, where this enzyme catalyzes the reduction of the delta-24 double bond of sterol intermediates such as desmosterol, during cholesterol biosynthesis. In addition, Seladin 1 has an anti-apoptotic function by reducing caspase 3 activity during oxidative stress-induced apoptosis and protecting cells against amyloid-beta peptide-induced apoptosis. Several studies have identified a reduction in expression levels of this enzyme in brain regions affected by Alzheimer's disease (hippocampus and the subventricular zone).

Subunit:
Endoplasmic reticulum membrane; Single-pass membrane protein. Golgi apparatus membrane; Single-pass membrane protein.

Subcellular Location:
Endoplasmic reticulum membrane; Single-pass membrane protein. Golgi apparatus membrane; Single-pass membrane protein.

Tissue Specificity:
Highly expressed in brain and adrenal gland with moderate expression in liver, lung, spleen, prostate and spinal cord. Low expression in heart, uterus and prostate. Undetectable in blood cells. In the brain, strongly expressed in cortical regions, substantia nigra, caudate nucleus, hippocampus, medulla oblongata and pons. In brains affected by Alzheimer disease, expression in the inferior temporal lobe is substantially lower than in the frontal cortex.

Similarity:
Belongs to the FAD-binding oxidoreductase/transferase type 4 family.
Contains 1 FAD-binding PCMH-type domain.

SWISS:
Q15392

Gene ID:
1718

Database links:

Entrez Gene: 1718 Human

Entrez Gene: 74754 Mouse

Entrez Gene: 298298 Rat

Omim: 606418 Human

SwissProt: Q15392 Human

SwissProt: Q8VCH6 Mouse

SwissProt: Q5BQE6 Rat

Unigene: 498727 Human

Unigene: 133370 Mouse

Unigene: 225146 Rat



Picture

Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Seladin 1) Polyclonal Antibody, Unconjugated (SL5390R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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