ADAMTS proteases are secreted enzymes containing a prometalloprotease domain of the reprolysin type. The ADAMTS proteases function in processing of procollagens and von Willebrand factor as well as catabolism of aggrecan, versican and brevican. They have important roles in connective tissue organization, coagulation, inflammation, arthritis, angiogenesis and cell migration.
Subcellular Location:
Secreted, extracellular space, extracellular matrix (By similarity).
Tissue Specificity:
Expressed in fetal liver and kidney, but not in any of the adult tissues examined.
Post-translational modifications:
The precursor is cleaved by a furin endopeptidase (By similarity).
Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be SLCglycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion (By similarity).
Similarity:
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Contains 3 TSP type-1 domains.
SWISS:
Q8TE58
Gene ID:
170689
Database links:
Entrez Gene: 170689 Human
Entrez Gene: 235130 Mouse
Entrez Gene: 300474 Rat
Omim: 607509 Human
SwissProt: Q8TE58 Human
SwissProt: P59384 Mouse
SwissProt: Q504Z2 Mouse
Unigene: 534221 Human
Unigene: 65867 Mouse
细胞外基质蛋白
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Sample:
Lane 1: Mouse Embryo tissue lysates
Lane 2: Mouse Placenta tissue lysates
Lane 3: Mouse Heart tissue lysates
Lane 4: Human MCF-7 cell lysates
Lane 5: Human 293T cell lysates
Primary: Anti-ADAMTS15 (SL5857R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 103 kDa
Observed band size: 100 kDa
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