E3 ubiquitin-protein ligase MARCH5; MARCH-V; Membrane-associated RING finger protein 5; Membrane-associated RING-CH protein V; RING finger protein 153.
Cat:
SL9339R
Species Reactivity:
(predicted: Human,Mouse,Rat,Chicken,Dog,Cow,)
Immunogen:
KLH conjugated synthetic peptide derived from human MARCH5:1-100/278
Format:
Liquid
Storage instructions:
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration:
1mg/ml
Clonality:
Polyclonal
Isotype:
IgG
Applications:
ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:50-200(Paraffin sections need to do antigen repair)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
MARCH5 is a member of the E3 ubiquitin-protein ligase family. This mitochonrial E3 ubiquitin-protein ligase plays an importnat role in controlling the morphology of the mitochondria. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. MARCH5 has been shown to interact with MTFN2 and ubiquitinated forms of DRP1.
Function: Mitochondrial E3 ubiquitin-protein ligase that plays a crucial role in the control of mitochondrial morphology by acting as a positive regulator of mitochondrial fission. May play a role in the prevention of cell senescence acting as a regulator of mitochondrial quality control. Promotes ubiquitination of FIS1, DNM1L and MFN1.
Subunit: Monomer and homodimer. Interacts with MFN1, MFN2, DNM1L and FIS1.
Subcellular Location: Mitochondrion outer membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane. Note=Authors show that the protein can be detected in endoplasmic reticulum (PubMed:14722266). Authors (PubMed:16874301) show its presence only in mitochondria (PubMed:16874301).
Tissue Specificity: Expressed in brain, heart, liver, lung, spleen, stomach, testis, skeletal and muscle.
Post-translational modifications: Autoubiquitinated leading to degradation (short half-life).
Specific References (1) | SL9339R has been referenced in 1 publications.
[IF=8.59] Hu et al. MARCH5 RNA promotes autophagy, migration, and invasion of ovarian cancer cells. (2017) Autophagy. 13:333-344 IHSLCP,IF(ICC) ; Human.