Rabbit Anti-ZWILCH antibody

Home > Product > Antibody > Rabbit Anti-ZWILCH antibody

hZwilch; KNTC1AP; MGC111034; Protein zwilch homolog; ZWILC_HUMAN; zwilch;y ZWILCH protein; Zwilch, kinetochore associated, homolog.

Cat:

SL9732R

Species Reactivity：

(predicted: Human,Mouse,Rat,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human ZWILCH:501-591/591

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:50-200（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

prev next

Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

Zwilch is the human homolog of the Drosophila Zwilch protein. The Drosophila Zwilch forms a complex with both ROD Rough Deal) and ZWINT (Zeste-White 10, also designated ZW10) proteins. This complex is important for chromosome segregation because it recruits cytoplasmic Dynein to the kinetochore and plays a crucial role in the spindle checkpoint. The role of Zwilch in complex is thought to be evolutionarily conserved because the human homologs of Zwilch, ZWINT and ROD coimmunoprecipitate in a human cell line called HeLa. The human Zwilch, ZWINT and ROD complex localizes to the kinetochores at prometaphase. Mutations were discovered in Zwilch, ZWINT and ROD during a screen for mutations in alleles encoding putative chromosome instability genes in cases of human colorectal cancer. These mutations may contribute in part to the chromosomal instability phenotype of colorectal tumor cells.

Function:
Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores.

Subunit:
Component of the RZZ complex composed of KNTC1/ROD, ZW10 and ZWILCH. Interacts directly with KNTC1/ROD. Component of the NRZ complex composed of ZWILCH, KNTC1/ROD, RINT1, ZW10 and NBAS.

Subcellular Location:
Chromosome, centromere, kinetochore.

SWISS:
Q9H900

Gene ID:
55055

Database links:

Entrez Gene: 55055 Human

Entrez Gene: 13614 Mouse

Omim: 609984 Human

SwissProt: Q9H900 Human

SwissProt: Q8R060 Mouse

Unigene: 21331 Human

Unigene: 335237 Mouse

Product Feedback Wall