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Home > Product > Antibody > Rabbit Anti-GRAF antibody
arhgap 26; ARHGAP26; FLJ42530; GRAF; GRAF1; GTPase regulator associated with focal adhesion kinase; GTPase regulator associated with focal adhesion kinase pp125(FAK); KIAA0621; oligophrenin 1 like protein; Oligophrenin-1-like protein; oligophrenin1like pr
Cat:
SL11248R
Species Reactivity：
Human,Mouse,Rat,(predicted: Chicken,Dog,Pig,Cow,Rabbit,Sheep,)
Immunogen：
KLH conjugated synthetic peptide derived from human GRAF1:1-100/814
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
WB=1:500-2000ELISA=1:5000-10000not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Sample: Cerebrum(Rat) Lysate at 40 ugCerebrum(Mouse) Lysate at 40 ugCerebellum(Rat) Lysate at 40 ugHepG2(Human) Cell Lysate at 30 ugKidney(Rat) Lysate at 40 ugKidney(Mouse) Lysate at 40 ugA549(Human) Cell Lysate at 30 ugA431(Human) Cell Lysate at 30 ugHL60(Human) Cell Lysate at 30 ugU937(Human) Cell Lysate at 30 ugCerebellum(Mouse) Lysate at 40 ugPrimary: Anti-GRAF (SL11248R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 36 kDObserved band size: 38 kD
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Price: $
Product PDFs
Datasheet:


Cellular signaling by G-proteins is down-regulated by GTPase-activating proteins (GAPs), which increase the rate of GTP hydroylsis. The GTPase regulator associated with focal adhesion kinase (GRAF) has GAP activity toward Rho A and Cdc42, but not Rac1. GRAF is ubiquitously expressed with high levels in heart and brain. Expression of GRAF causes clearing of stress fibers and formation of long actin based filopodial-like extensions. Fusion of MLL with GRAF, MLL/GRAF, is included in a rare genetic subgroup of acute myeloid leukemia (AML) cases.

Function:
GTPase-activating protein for RHOA and CDC42.

Subunit:
Interacts with NYAP1, NYAP2 and MYO16 (By similarity). Binds to the SLCterminus of PTK2/FAK1.

Subcellular Location:
Cell junction; focal adhesion. Cytoplasm; cytoskeleton. Colocalizes with actin stress fibers and cortical actin structures.

DISEASE:
Defects in ARHGAP26 are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia. Chromosomal translocation t(5;11)(q31;q23) with MLL has been found in a JMML patient.

Similarity:
Contains 1 PH domain. Contains 1 Rho-GAP domain. Contains 1 SH3 domain.

SWISS:
Q9UNA1

Gene ID:
23092

Database links:

Entrez Gene: 23092 Human

Entrez Gene: 71302 Mouse

Entrez Gene: 307459 Rat

Omim: 605370 Human

SwissProt: Q9UNA1 Human

SwissProt: Q6ZQ82 Mouse

Unigene: 654668 Human

Unigene: 329396 Mouse

Unigene: 214162 Rat



Picture

Sample:
Cerebrum(Rat) Lysate at 40 ug
Cerebrum(Mouse) Lysate at 40 ug
Cerebellum(Rat) Lysate at 40 ug
HepG2(Human) Cell Lysate at 30 ug
Kidney(Rat) Lysate at 40 ug
Kidney(Mouse) Lysate at 40 ug
A549(Human) Cell Lysate at 30 ug
A431(Human) Cell Lysate at 30 ug
HL60(Human) Cell Lysate at 30 ug
U937(Human) Cell Lysate at 30 ug
Cerebellum(Mouse) Lysate at 40 ug
Primary: Anti-GRAF (SL11248R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 38 kD
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