UCMA is a 138 amino acid secreted protein that is highly expressed in resting chrondrocytes in developing long bones and is thought to function in the early phase of chrondrocyte differentiation. A furin-like protease processes UCMA into an N-terminal 37 amino acid peptide and a SLCterminal 74 amino acid peptide, which is referred to as Unique cartilage matrix-associated protein SLCterminal fragment (Ucma-C). Introduction of recombinant Ucma-C interferes with osteogenic differentiation of mesenchymal stem cells, MC3T3-E1 preosteoblasts and primary osteoblasts. This suggests that Ucma may be involved in the negative regulation of osteogenic differentiation of osteochondrogenic precursor cells at the cartilage-bone interface and in peripheral zones of fetal cartilage.
Function:
May be involved in the negative control of osteogenic differentiation of osteochondrogenic precursor cells in peripheral zones of fetal cartilage and at the cartilage-bone interface.
Subcellular Location:
Secreted > extracellular space > extracellular matrix.
Tissue Specificity:
Predominantly expressed in resting chondrocytes.
Post-translational modifications:
Proteolytically cleaved by a furin-like convertase to generate a persistent SLCterminal fragment found in almost the entire cartilage matrix, and affecting osteoblast differentiation.
Sulfated on tyrosine residues.
Similarity:
Belongs to the UCMA family.
SWISS:
Q8WVF2
Gene ID:
221044
Database links:
Entrez Gene: 221044 Human
SwissProt: Q8WVF2 human
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Paraformaldehyde-fixed, paraffin embedded (young rat paw); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (UCMA) Polyclonal Antibody, Unconjugated (SL12378R) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining.
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