Gas2 is a 313 amino acid protein encoded by the human gene GAS2. Gas2 is thought to play a role in apoptosis by acting as a cell death substrate for caspases. Gas2, a component of the microfilament system, is cleaved by a caspase (caspase-3 and caspase-7) at Asparagine 278 during apoptosis. The cleaved form resulting from this dramatically induces the rearrangement of the Actin cytoskeleton and causes potent changes in the shape of the affected cells. Gas2 is believed to also be involved in the membrane ruffling process. During the G0-G1 transition phase Gas2 can be found phosphorylated on its serine residues. Gas2 is a cytoskeleton and peripheral membrane protein that co-localizes with Actin fibers at the cell border and along the stress fibers in growth-arrested fibroblasts. Gas2 is mainly membrane-associated but when hyperphosphorylated it will accumulate at membrane ruffles. Gas2 is specifically expressed at growth arrest and is ubiquitously expressed with highest levels found in liver, lung and kidney. There is no evidence, however, of Gas2 expression in spleen.
Function:
May play a role in apoptosis by acting as a cell death substrate for caspases. Is cleaved during apoptosis and the cleaved form induces dramatic rearrangements of the actin cytoskeleton and potent changes in the shape of the affected cells. May be involved in the membrane ruffling process.
Subcellular Location:
Cytoplasm > cytoskeleton. Membrane. Component of the microfilament system. Colocalizes with actin fibers at the cell border and along the stress fibers in growth-arrested fibroblasts. Mainly membrane-associated. When hyperphosphorylated, accumulates at membrane ruffles.
Tissue Specificity:
Ubiquitously expressed with highest levels in liver, lung, and kidney. Not found in spleen.
Post-translational modifications:
Cleaved, during apoptosis, on a specific aspartic residue by caspases.
Phosphorylated on serine residues during the G0-G1 transition phase.
Similarity:
Belongs to the GAS2 family.
Contains 1 CH (calponin-homology) domain.
Contains 1 GAR domain.
SWISS:
O43903
Gene ID:
2620
Database links:
Entrez Gene: 2620 Human
Entrez Gene: 14453 Mouse
Omim: 602835 Human
SwissProt: O43903 Human
SwissProt: P11862 Mouse
Unigene: 632151 Human
Unigene: 20772 Mouse
Unigene: 386823 Mouse
Unigene: 471773 Mouse
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