Endonuclease which cleaves flap structures at the junction between single-stranded DNA and double-stranded DNA. Specific for 5'-overhanging flap structures in which the 5'-upstream of the flap is completely double-stranded. Prefers the blocked-flap structures similar to those occurring at replication forks, in which the 5' single-strand overhang of the flap is double-stranded.
Function:
Endonuclease which resolves Holliday junctions by the introduction of symmetrically related cuts across the junction point, to produce nicked duplex products in which the nicks can be readily ligated. Four-way DNA intermediates, also known as Holliday junctions, are formed during homologous recombination and DNA repair, and their resolution is necessary for proper chromosome segregation.
Subcellular Location:
Nucleus.
Similarity:
Belongs to the XPG/RAD2 endonuclease family. GEN subfamily.
SWISS:
Q17RS7
Gene ID:
348654
Database links:
Entrez Gene: 348654 Human
Entrez Gene: 298884 Rat
Omim: 612449 Human
SwissProt: Q17RS7 Human
Unigene: 467793 Human
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