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Home > Product > Antibody > Rabbit Anti-phospho-SLP76 (Tyr113) antibody
SLP76 (phospho Y113); p-SLP76 (Y113); p-LCP2; 76 kDa tyrosine phosphoprotein; CG8697; LCP 2; LCP 2; LCP2; LCP2_HUMAN; Lymphocyte Cytosolic Protein 2; Lymphocyte Cytosolic Protein 2; SH2 Domain Containing Leukocyte Protein 76 KD; SH2 domain containing leuk
Cat:
SL13663R
Species Reactivity：
Human,Mouse,(predicted: Pig,Cow,Horse,Rabbit,Sheep,)
Immunogen：
KLH conjugated Synthesised phosphopeptide derived from human SLP76 around the phosphorylation site of Tyr113:DD(p-Y)ES
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
WB=1:500-2000ELISA=1:5000-10000not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Sample: Jurkat(Human) Cell Lysate at 30 ugSpleen (Mouse) Lysate at 40 ugPrimary: Anti-phospho-SLP76 (Tyr113) (SL13663R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 75 kDObserved band size: 75 kD
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Product PDFs
Datasheet:


The translational product of the Vav proto-oncogene is exclusively expressed in cells of hematopoietic origin and is critical for lymphocyte development and activation. However, the biochemical basis of Vav’s function is unclear. Vav contains a single SH2 domain that is required for its association with the T cell receptor (TCR). Overexpression of Vav or SLP-76 in Jurkat cells leads to NFAT activation and IL-2 production. When co-expressed, Vav and SLP-76 synergize to induce a robust basal and TCR-mediated IL-2 response. Although SLP-76 does not contain a motif that would indicate it to be a member of the tyrosine, serine/threonine or lipid kinase families, it does contain several putative SH2/SH3-binding domains and has been shown to physically associate with the adapter protein GRB2 as well as PLC g1. The discovery of SLP-76 represents an important step in elucidating the mechanism of Vav transformation and TCR-mediated NFAT activation.

Function:
Involved in T-cell antigen receptor mediated signaling.

Subunit:
Interacts with SLA. Interacts with CBLB (By similarity). Interacts with the adapter proteins GRB2 and FYB. Interacts with SHB. Interacts with PRAM1.

Subcellular Location:
Cytoplasm.

Tissue Specificity:
Highly expressed in spleen, thymus and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblastoma cell lines. Post-translational modifications : Phosphorylated after T-cell receptor activation by ZAP-70.

Similarity:
Contains 1 SAM (sterile alpha motif) domain.
Contains 1 SH2 domain.

SWISS:
Q13094

Gene ID:
3937

Database links:

Entrez Gene: 3937 Human

Omim: 601603 Human

SwissProt: Q13094 Human

Unigene: 304475 Human



Picture

Sample:
Jurkat(Human) Cell Lysate at 30 ug
Spleen (Mouse) Lysate at 40 ug
Primary: Anti-phospho-SLP76 (Tyr113) (SL13663R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 75 kD
Observed band size: 75 kD
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