NOSTRIN (nitric oxide synthase trafficker isoform 1), also known as endothelial nitric oxide synthase traffic inducer, is a member of the Pombe Cdc15 homology (PCH) family of proteins. NOSTRIN is expressed in the vascular endothelial cells of highly vascularized tissues such as placenta, lung, kidney and heart. It consists of an N-terminal Cdc15 domain with an FCH (Fes/CIP homology) region, two coiled coil domains and a SLCterminal SH3 domain. NOSTRIN typically exists as a trimer. It functions as an adaptor protein binding to caveolin-1 via an internal domain and NOS3 via its SH3 domain, forming a ternary complex which facilitates caveolar transport of NOS3. The NOS3 protein is responsible for the production of nitric oxide (NO), a potent mediator in various biological processes. The translocation of NOS3 from the plasma membrane to intracellular vesicle-like structures diminishes NO production. NOSTRIN also interacts with Dynamin and N-WASP via its SH3 domain.
Function:
Multivalent adapter protein which may decrease NOS3 activity by inducing its translocation away from the plasma membrane.
Subcellular Location:
Cell membrane. Cytoplasmic vesicle. Cytoplasm > cytoskeleton. Enriched in selected actin structures.
Tissue Specificity:
Expressed at highest levels in heart, kidney, placenta and lung, and at lowest levels in brain, thymus and spleen. Present in vascular endothelial cells and placenta. Over-expressed in placenta from women with pre-eclampsia (at protein level).
Similarity:
Contains 1 FCH domain.
Contains 1 SH3 domain.
SWISS:
Q8IVI9
Gene ID:
115677
Database links:
Entrez Gene: 115677 Human
Entrez Gene: 329416 Mouse
Omim: 607496 Human
SwissProt: Q8IVI9 Human
SwissProt: Q6WKZ7 Mouse
Unigene: 189780 Human
Unigene: 90047 Mouse
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