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Home > Product > Antibody > Rabbit Anti-TOMM20 antibody
TOM20; KIAA0016; MAS20; MGC117367; Mitochondrial 20 kDa outer membrane protein; Mitochondrial import receptor subunit TOM20 homolog; MOM19; Outer mitochondrial membrane receptor Tom20; TOM 20; TOM20_HUMAN; Translocase of outer mitochondrial membrane 20 ho
Cat:
SL7357R
Species Reactivity：
Human,Mouse,Rat,(predicted: Dog,Cow,Horse,Rabbit,Sheep,)
Immunogen：
KLH conjugated synthetic peptide derived from human TOM20:1-100/145
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Sample: Lane 1: Mouse Kidney Lysates Lane 2: Mouse Brain Lysates Lane 3: Mouse Heart Lysates Lane 4: Mouse NIH/3T3 Lysates Lane 5: Rat Kidney Lysates Lane 6: Rat Brain Lysates Lane 7: Rat Heart Lysates Lane 8: Human Hela cell Lysates Lane 9: Human THP-1 cell Lysates Lane 10: Human HL-60 cell Lysates Lane 11: Human 293T cell Lysates Primary: Anti-TOMM20 (SL7357R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 16kDaObserved band size: 16kDa
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Product PDFs
Datasheet:


The mitochondrial preprotein translocases of the outer membrane (Tom) is a multisubunit protein complex that facilitates the import of nucleus-encoded precursor proteins across the mitochondrial outer membrane (1). The Tom machinery consists of import receptors for the initial binding of cytosolically synthesized preproteins and a general import pore (GIP) for the membrane translocation of various preproteins into the mitochondria (2). The import receptors include Tom20 and Tom22, which form a heteromeric receptor complex that initiates the insertion of newly synthesized proteins into the outer membrane and then directs the precursor protein into the GIP (3,4). In yeast, Tom22 is the essential component of the import receptor complex as it functions as both a receptor for the preproteins and serves as a docking point for both Tom20 and the GIP (5,6). Tom22 directly associates with Tom40, the major component of the GIP, and thereby forms a stable interaction between the two core complexes to facilitate the fluid movement of preproteins into the mitochondria (6,7). The insertion of Tom40 into the Tom machinery requires the initial binding of Tom40 to Tom20 and leads to the efficient incorporation of Tom40 precursors into preexisting Tom complexes (2,8)

Function:
Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore.

Subunit:
Forms part of the preprotein translocase complex of the outer mitochondrial membrane (TOM complex).

Subcellular Location:
Mitochondrion outer membrane.

Similarity:
Belongs to the Tom20 family.

SWISS:
Q15388

Gene ID:
1964

Database links:
Entrez Gene: 1964 Human

Entrez Gene: 67952 Mouse

Entrez Gene: 266601 Rat

SwissProt: Q15388 Human

SwissProt: Q9DCC8 Mouse

SwissProt: Q62760 Rat



Picture

Sample:
Lane 1: Mouse Kidney Lysates
Lane 2: Mouse Brain Lysates
Lane 3: Mouse Heart Lysates
Lane 4: Mouse NIH/3T3 Lysates
Lane 5: Rat Kidney Lysates
Lane 6: Rat Brain Lysates
Lane 7: Rat Heart Lysates
Lane 8: Human Hela cell Lysates
Lane 9: Human THP-1 cell Lysates
Lane 10: Human HL-60 cell Lysates
Lane 11: Human 293T cell Lysates
Primary: Anti-TOMM20 (SL7357R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 16kDa
Observed band size: 16kDa
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