ACAP1 is a member of the ADP ribosylation factor family of ARF6 GTPase-activating proteins (GAP). GAPs are important regulators of Arf function by controlling the return of ARF to its inactive state. ACAP1 is related to AGAP1 and ASAP1, and all three proteins are similarly expressed in fibroblast cells such as NIH/3T3. Internalization and recycling of integrin receptors is important in cell adhesion and migration modulation, and once inside a cell, proteins and membranes are transported to the endosome where they are sorted for recycling or degradation. ACAP1 promotes cargo sorting by associating directly to recycling cargo proteins. Preventing this interaction inhibits protein recycling. ACAP1 binds transferrin receptors, promoting their transport to the plasma membrane from the endosome. Akt induced phosphorylation of ACAP1 at Ser 554 regulates ACAP1 interaction to integrin in endosomes, and downregulation of Akt or ACAP1 may inhibit cell migration on Fibronectin.
Function:
GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface.
Tissue Specificity:
Highest level in lung and spleen. Low level in heart, kidney, liver and pancreas.
Post-translational modifications:
Phosphorylation at Ser-554 by PKB is required for interaction with ITGB1, export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.
Similarity:
Contains 3 ANK repeats.
Contains 1 Arf-GAP domain.
Contains 1 BAR domain.
Contains 1 PH domain.
SWISS:
Q15027
Gene ID:
9744
Database links:
Entrez Gene: 9744 Human
Entrez Gene: 216859 Mouse
Omim: 607763 Human
SwissProt: Q15027 Human
SwissProt: Q8K2H4 Mouse
Unigene: 337242 Human
Unigene: 288671 Mouse
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