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Rabbit Anti-GTF2F1 antibody
In eukaryotic systems, initiation of transcription from protein-coding genes is a complex process requiring RNA polymerase II and broad families of auxiliary transcription factors. Such factors can be divided into two major functional classes: the basal factors that are required for transcription of all Pol II genes, including TFIIA, TFIIB, TFIID, TFIIE, TFIIF and TFIIH; and sequence-specific factors that regulate gene expression. The basal transcription factors and Pol II form a specific multiprotein complex near the transcription start site by interacting with core promotor elements such as the TATA box generally located 25-30 base pairs upstream of the transcription start site. TFIIF, a heteromer composed of a small (RAP 30) and a large (RAP 74) subunit, is required for RNA polymerase II to assemble into a preinitiation complex formed by promotor DNA and the general factors TFIID, IIA and IIB. In addition, TFIIF stimulates transcription elongation by RNA polymerase II.
Function:
The RNA polymerase II transcription factor TFIIF is a heterodimer with 2 subunits: one, referred to as RAP74, of approximately 70 kD and the other, known as RAP30 or GTF2F2, of 30 kD. TFIIF affects RNA polymerase II activity both during the initiation and elongation stages of RNA transcription. TFIIF appears to assist TFIIB in recruiting RNA polymerase II into a preinitiation complex. It also affects RNA transcription by suppressing transient pausing of the polymerase at locations on the DNA template. TFIIF mediates the association of RNA polymerase II with promoter sequences containing transcription factors IID, IIB, and IIA (DAB complex). Cloned human RAP 30 was sufficient for the recruitment of RNA polymerase II to the DAB complex. This ability of RAP 30 to recruit RNA polymerase to a promoter is also a characteristic of sigma factors in prokaryotes.
Subunit:
Heterodimer of an alpha and a beta subunit. Interacts with HTATSF1 and GPBP1 (By similarity). Interacts with URI1.
Subcellular Location:
Nuclear
Similarity:
Belongs to the TFIIF beta subunit family.
SWISS:
P13984
Gene ID:
2962
Database links:
Entrez Gene: 2962 Human
Entrez Gene: 505702 Cow
Entrez Gene: 2963 Human
Entrez Gene: 68705 Mouse
Entrez Gene: 19653 Mouse
Entrez Gene: 316123 Rat
Entrez Gene: 81674 Rat
Omim: 189968 Human
Omim: 189969 Human
SwissProt: Q5EA53 Cow
SwissProt: P13984 Human
SwissProt: P35269 Human
SwissProt: Q8BVJ2 Mouse
SwissProt: Q8R5B7 Mouse
SwissProt: Q01750 Rat
SwissProt: Q6AY96 Rat
Unigene: 654582 Human
Unigene: 68257 Human
Unigene: 24632 Mouse
Unigene: 98836 Rat
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