Rabbit Anti-phospho-MCM2 (Ser53) antibody

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MCM4 (phospho S53); MCM2(phospho-Ser53); MCM2(phospho Ser53); MCM2 (phospho S54); p-MCM2(Ser53); p-MCM2(S53); rat MCM4 (phospho S54); BM28; CCNL 1; CCNL1; CDC like 1; CDC like-1; cdc19; CDCL 1; CDCL1; Cell devision cycle like 1; Cyclin like 1; cyclin like

Cat:

SL10608R

Species Reactivity：

Human,(predicted: Mouse,Rat,Chicken,Dog,Pig,Horse,Rabbit,Zebrafish,Sheep,)

Immunogen：

KLH conjugated Synthesised phosphopeptide derived from human MCM2 around the phosphorylation site of Ser53:DE(p-S)EG

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: MCF-7 (Human) Cell Lysate at 30 ugLane 2: Jurkat (Human) Cell Lysate at 30 ugLane 3: HepG2 (Human) Cell Lysate at 30 ugLane 4: U-2OS (Human) Cell Lysate at 30 ugPrimary: Anti- phospho-MCM2 (Ser53) (SL10608R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 120 kDObserved band size: 120 kD

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Unit:

Price: $256.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Oct 2012]

Function:
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.

Subunit:
Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5. Interacts with KAT7 and DSF4. May interact with MCM10.

Subcellular Location:
Nucleus (Probable).

Post-translational modifications:
Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DSF4 and CDC7-DSF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DSF4 complex. Phosphorylation by the CDC7-DSF4 complex during G1/S phase is required for the initiation of DNA replication.

Similarity:
Belongs to the MCM family.
Contains 1 MCM domain.

SWISS:
P49736

Gene ID:
4171

Database links:

Entrez Gene: 4171 Human

Entrez Gene: 17216 Mouse

Entrez Gene: 312538 Rat

Omim: 116945 Human

SwissProt: P49736 Human

SwissProt: P97310 Mouse

Unigene: 477481 Human

Unigene: 16711 Mouse

Unigene: 2715 Rat

Picture

Sample:
Lane 1: MCF-7 (Human) Cell Lysate at 30 ug
Lane 2: Jurkat (Human) Cell Lysate at 30 ug
Lane 3: HepG2 (Human) Cell Lysate at 30 ug
Lane 4: U-2OS (Human) Cell Lysate at 30 ug
Primary: Anti- phospho-MCM2 (Ser53) (SL10608R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 120 kD
Observed band size: 120 kD

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