The tripartite motif (TRIM) family of proteins are characterized by a conserved TRIM domain that includes a coiled-coil region, a B-box type zinc finger, one RING finger and three zinc-binding domains. TRIM72 (tripartite motif containing 72), also known as MG53, is a 477 amino acid cytoplasmic vesicle membrane protein that belongs to the TRIM/RBCC family. Existing as a homooligomer, TRIM72 contains one B box-type zinc finger, one B30.2/SPRY domain and a RING-type zinc finger. TRIM72 is considered a muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. TRIM72 is required for transport of dysferlin to sites of cell injury during repair patch formation. TRIM72 also regulates membrane budding and exocytosis and may be involved in the regulation of the mobility of KV2.1-containing endocytic vesicles. TRIM72 exists as two alternatively spliced isoforms and is encoded by a gene located on human chromosome 16p11.2.
Function:
Muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Specifically binds phosphatidylserine. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca(2+)-dependent membrane resealing process. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membrane budding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles.
Subcellular Location:
Cell membrane > sarcolemma. Cytoplasmic vesicle membrane. Tethered to plasma membrane and cytoplasmic vesicles via its interaction with phosphatidylserine.
Post-translational modifications:
Disulfide bond formation at Cys-242 occurs in case of membrane damage that cause the entry of the oxidized milieu of the extracellular space, resulting in homooligomerization.
Similarity:
Belongs to the TRIM/RBCC family.
Contains 1 B box-type zinc finger.
Contains 1 B30.2/SPRY domain.
Contains 1 RING-type zinc finger.
SWISS:
Q6ZMU5
Gene ID:
493829
Database links:
Entrez Gene: 493829 Human
Entrez Gene: 434246 Mouse
Entrez Gene: 100008639 Rabbit
Entrez Gene: 365377 Rat
SwissProt: Q6ZMU5 Human
SwissProt: Q1XH17 Mouse
SwissProt: Q1XH18 Rabbit
SwissProt: A0JPQ4 Rat
Unigene: 661859 Human
Unigene: 389924 Mouse
Unigene: 23565 Rat
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