The protein encoded by this gene is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of the protein with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors. This gene encodes the 25kD subunit of the protein complex, which is composed of four polypeptides. [provided by RefSeq, Jul 2008]
Function:
Component of the cleavage factor Im (CFIm) complex that plays a key role in pre-mRNA 3'-processing. Involved in association with CPSF6 or CPSF7 in pre-MRNA 3'-end poly(A) site cleavage and poly(A) addition. NUDT21/CPSF5 binds to cleavage and polyadenylation RNA substrates. The homodimer mediates simultaneous sequence-specific recognition of two 5'-UGUA-3' elements within the pre-mRNA. Binds to, but does not hydrolyze mono- and di-adenosine nucleotides. May have a role in mRNA export.
Subcellular Location:
Nucleus. In punctate subnuclear structures localized adjacent to nuclear speckles, called paraspeckles.
Post-translational modifications:
Acetylated mainly by p300/CBP, recruited to the complex by CPSF6. Acetylation decreases interaction with PAPAO. Deacetylated by the class I/II HDACs, HDAC1, HDAC3 and HDAC10, and by the class III HDACs, SIRT1 AND SIRT2.
Similarity:
Belongs to the Nudix hydrolase family. CPSF5 subfamily.
Contains 1 nudix hydrolase domain.
SWISS:
O8769
Gene ID:
11051
Database links:
Entrez Gene: 11051 Human
Entrez Gene: 68219 Mouse
Entrez Gene: 291877 Rat
Entrez Gene: 394092 Zebrafish
SwissProt: Q3ZCA2 Cow
SwissProt: O8769 Human
SwissProt: Q9CQF3 Mouse
SwissProt: Q4KM65 Rat
SwissProt: Q7T3C6 Zebrafish
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