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Home > Product > Antibody > Rabbit Anti-ATG14 antibody
4832427M01; ATG14; Atg14L; Autophagy-related protein 14-like protein; BAKOR_HUMAN; Barkor; Beclin 1-associated autophagy-related key regulator; D14Ertd114e; D14Ertd436e; KIAA0831; mCG_6911.
Cat:
SL7462R
Species Reactivity：
(predicted: Human,Mouse,Rat,Dog,Pig,Cow,)
Immunogen：
KLH conjugated synthetic peptide derived from human ATG14:41-140/492
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ATG14) Polyclonal Antibody, Unconjugated (SL7462R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Product PDFs
Datasheet:


Required for both basal and inducible autophagy. Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Promotes BECN1 translocation from the trans-Golgi network to autophagosomes. Enhances PIK3C3 activity in a BECN1-dependent manner.

Function:
Required for both basal and inducible autophagy. Determines the localization of the autophagy-specific PI3-kinase complex. Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Promotes BECN1 translocation from the trans-Golgi network to autophagosomes. Enhances PIK3C3 activity in a BECN1-dependent manner.

Subunit:
Component of the autophagy-specific PI3-kinase complex I composed of ATG14, BECN1, PIK3C3 and PIK3R4, but not UVRAG, nor KIAA0226/Rubicon. UVRAG and ATG14/Barkor form mutually exclusive complexes with BECN1 through direct competition. The complex containing ATG14 up-regulates autophagy, while the one containing Rubicon down-regulates autophagy (By similarity). Interacts with PIK3CB (By similarity). Interacts with BECN1P1/BECN2.

Subcellular Location:
Cytoplasm. Endoplasmic reticulum. Cytosolic under nutrient-rich conditions. Following autophagy stimuli, such as starvation or rapamycin induction, predominantly detected in cytoplasmic foci, identified as isolation membranes and autophagosomes.

Similarity:
Belongs to the Barkor family.

SWISS:
Q6ZNE5

Gene ID:
22863

Database links:

Entrez Gene: 22863 Human

Entrez Gene: 100504663 Mouse

Entrez Gene: 305831 Rat

Omim: 613515 Human

SwissProt: Q6ZNE5 Human

SwissProt: Q8CDJ3 Mouse

SwissProt: D4A4K3 Rat

Unigene: 42969 Human

Entrez Gene: 22863 Human

  • Entrez Gene: 100504663 Mouse
  • Entrez Gene: 305831 Rat
  • Omim: 613515 Human
  • SwissProt: Q6ZNE5 Hun
  • SwissProt: Q8CDJ3 MouseSwissProt: D4A4K3 Rat
  • Unigene: 42969 Human


    • Picture

    Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ATG14) Polyclonal Antibody, Unconjugated (SL7462R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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