[email protected]
Home > Product > Antibody > Rabbit Anti-HMG4/HMGB3 antibody
High mobility group (nonhistone chromosomal) protein 4; High mobility group box 3; High mobility group protein 2a; High mobility group protein 4; High mobility group protein B3; High mobility group protein HMG4; HMG 4; HMG-2a; HMG-4; HMG2A; HMGB
Cat:
SL1769R
Species Reactivity：
Mouse,Rat,(predicted: Human,Chicken,Dog,Pig,Cow,Horse,Rabbit,)
Immunogen：
KLH conjugated synthetic peptide derived from human HMG4/HMGB3:1-100/200
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Paraformaldehyde-fixed, paraffin embedded (mouse bladder); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HMG4) Polyclonal Antibody, Unconjugated (SL1769R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Rat bladder); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HMG4) Polyclonal Antibody, Unconjugated (SL1769R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
More
prev next
Unit:
Price: $
Product PDFs
Datasheet:


HMGB3 belongs to the high mobility group (HMG) protein superfamily. Like HMG1 (MIM 163905) and HMG2 (MIM 163906), HMGB3 contains DNA-binding HMG box domains and is classified into the HMG box subfamily. Members of the HMG box subfamily are thought to play a fundamental role in DNA replication, nucleosome assembly and transcription (Wilke et al., 1997 [PubMed 9370291]; Nemeth et al., 2006 [PubMed 16945912]).[supplied by OMIM, Mar 2008]

Function:
Binds preferentially single-stranded DNA and unwinds double stranded DNA.

Subcellular Location:
Nucleus. Chromosome.

Tissue Specificity:
Expressed predominantly in placenta.

Similarity:
Belongs to the HMGB family.
Contains 2 HMG box DNA-binding domains.

SWISS:
O15347

Gene ID:
3149

Database links:

Entrez Gene: 3149 Human

Entrez Gene: 613729 Cow

Entrez Gene: 15354 Mouse

Entrez Gene: 305373 Rat

Omim: 300193 Human

SwissProt: Q32L31 Cow

SwissProt: O15347 Human

SwissProt: O54879 Mouse

Unigene: 19114 Human

Unigene: 37287 Mouse



Picture

Paraformaldehyde-fixed, paraffin embedded (mouse bladder); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HMG4) Polyclonal Antibody, Unconjugated (SL1769R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat bladder); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HMG4) Polyclonal Antibody, Unconjugated (SL1769R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Product Feedback Wall
Message :
Your Email :
Copyright © 2007-2018 Sunlong Medical All Rights Reserved.