The high-mobility group (HMG) proteins 14 and 17 are abundant chromosomal proteins that bind to nucleosomes and enhance transcription (1–5). HMG-14 and HMG-17 also function as architectural elements, which alter the structure of the chromatin fiber and enhance transcription from chromatin templates (1–3,5). HMG-14/17 proteins modify the nucleosomal organization of the 30 nm chromatin fiber and mediate the unfolding of the higher order chromatin structure thereby facilitating access to the underlying DNA sequence (1–3). Clustering of architectural elements, such as HMG proteins and linker histone subtypes into distinct domains, may lead to structural and functional heterogeneity along the chromatin fiber (1–3). In addition, HMG-14 and HMG-17 have been identified as constitutive components of mouse oocyte and embryonic chromatin that establish a link between the structure of embryonic chromatin and the normal progression of embryonic development (2).
Function:
Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in an unique chromatin conformation.
Subcellular Location:
Nucleus. Cytoplasm. Cytoplasmic enrichment upon phosphorylation.
Post-translational modifications:
Phosphorylation favors cytoplasmic localization.
Similarity:
Belongs to the HMGN family.
SWISS:
P05204
Gene ID:
3151
Database links:
Entrez Gene: 3151 Human
Clone ID: BC014644 Human
Entrez Gene: 10004485 Mouse
Entrez Gene: 100503799 Mouse
Entrez Gene: 15331 Mouse
Entrez Gene: 114637 Rat
IMAGE Id: 3355317 Human
Omim: 163910 Human
SwissProt: P05204 Human
SwissProt: P09602 Mouse
SwissProt: P18437 Rat
Unigene: 181163 Human
Unigene: 319660 Mouse
Unigene: 911 Mouse
Unigene: 3517 Rat
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Paraformaldehyde-fixed, paraffin embedded (rat skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HMGN2HMG17) Polyclonal Antibody, Unconjugated (SL3654R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HMGN2HMG17) Polyclonal Antibody, Unconjugated (SL3654R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HMGN2HMG17) Polyclonal Antibody, Unconjugated (SL3654R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HMGN2HMG17) Polyclonal Antibody, Unconjugated (SL3654R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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