Rabbit Anti-TOB antibody | Sunlong Medical

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APRO 6; APRO6; MGC104792; MGC34446; PIG 49; PIG49; Proliferation inducing gene 49; Protein Tob1; RP23 244C22.1; TOB 1; Tob 1 protein; TOB; TOB1; Tob1 protein; Transducer of ERBB 2 1; Transducer of erbB 2; Transducer of ERBB2 1; Transducer

Cat:

SL18164R

Species Reactivity：

Rat,(predicted: Human,Mouse,Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human TOB:131-230/345

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TOB) Polyclonal Antibody, Unconjugated (SL18164R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a member of the transducer of erbB-2 /B-cell translocation gene protein family. Members of this family are anti-proliferative factors that have the potential to regulate cell growth. The encoded protein may function as a tumor suppressor. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]

Function:
The Tob/Btg family of proteins consists of a large number of members. These proteins have a common domain in their amino terminal end and may have anti-proliferative activity in various cell types. The Tob protein was identified in a search for molecules that interact with the receptor tyrosine kinase ErbB2. Active ErbB2 has a negative effect on the anti-proliferative activity of Tob. However, Tob is phosphorylated on serine and threonine residues but not on tyrosine, suggesting that active ErbB2 activates a Ser/Thr kinase that phosphorylates Tob. Unphosphorylated Tob suppresses expression of cyclin D1. It was shown that active p90Rsk1 kinase (known to be activated by protein-tyrosine kinase receptor) phosphorylates Tob on serine and threonine residues in vitro. In addition, Erk1/Erk2 MAP kinases phosphorylate Tob in vivo and in vitro, resulting in suppression of the anti-proliferative activity of Tob. Homozygous Tob knockout mice develop greater bone mass resulting from increased numbers of osteoblasts. Furthermore, it has been shown in osteoblasts, that upon BMP2 (bone morphogenetic protein) activation, Tob associates with receptor regulated Smads (Smad 1, 5, and 8). Thus, osteoblast proliferation and differentiation is negatively regulated by Tob protein through the Smad proteins.

Subunit:
Interacts with ERBB2. Interacts with CNOT7 and CNOT8.

Tissue Specificity:
Ubiquitous.

Post-translational modifications:
Phosphorylated on Ser and Thr residues.

Similarity:
Belongs to the BTG family.

SWISS:
P50616

Gene ID:
10140

Database links:

Entrez Gene: 10140 Human

Entrez Gene: 22057 Mouse

Entrez Gene: 170842 Rat

Omim: 605523 Human

SwissProt: P50616 Human

SwissProt: Q61471 Mouse

SwissProt: Q8R5K6 Rat

Unigene: 531550 Human

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